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Home>Product>Primary Antibody

AKR1C4 Mouse mAb

#6997

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Western blotting analysis using AKR1C4 antibody (Cat#6997). Total lysates (30 μg) from various cell lines and rat liver tissue were loaded and separated by SDS-PAGE. The blot was incubated with AKR1C4 antibody (Cat#6997, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human hepatocarcinoma using AKR1C4 antibody (Cat#6997, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    AKR1C4
  • Catalog：
    6997
  • Application：
    WB, IHC-P
  • Species Reactivity：
    H

Information

Data

Information
Product Name AKR1C4 Mouse mAb
Aliases AKR1C4; Chlordecone Reductase; Aldo-Keto Reductase Family 1 Member C4; 3-Alpha-HSD1; CHDR; MGC22581; HAKRA; DD-4; DD4; Aldo-Keto Reductase Family 1, Member C4 (Chlordecone Reductase; 3-Alpha Hydroxysteroid Dehydrogenase, Type I; Dihydrodiol Dehydrogenase 4); CDR; Chlordecone Reductase; 3-Alpha Hydroxysteroid Dehydrogenase, Type I; Dihydrodiol Dehydrogenase 4; 3-Alpha-HSD; 3-Alpha Hydroxysteroid Dehydrogenase, Type I; C11; Type I 3-Alpha-Hydroxysteroid Dehydrogenase; 3-Alpha-Hydroxysteroid 3-Dehydrogenase Type I; 3alpha-Hydroxysteroid 3-Dehydrogenase; Dihydrodiol Dehydrogenase 4; Dihydrodiol Dehydrogenase Isozyme DD4
Background

Gene Name: AKR1C4

NCBI Gene Entry: 1109

UniProt Entry: P17516

Application Information

Molecular Weight: Predicted, 37 kDa; observed, 35 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 26GB2270

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunohistochemistry-paraffin (IHC-P)
Immunogen Recombinant protein of human AKR1C4
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.03% Proclin 300.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using AKR1C4 antibody (Cat#6997). Total lysates (30 μg) from various cell lines and rat liver tissue were loaded and separated by SDS-PAGE. The blot was incubated with AKR1C4 antibody (Cat#6997, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded human hepatocarcinoma using AKR1C4 antibody (Cat#6997, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    AKR1C4
  • Catalog：
    6997
  • Application：
    WB, IHC-P
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using AKR1C4 antibody (Cat#6997). Total lysates (30 μg) from various cell lines and rat liver tissue were loaded and separated by SDS-PAGE. The blot was incubated with AKR1C4 antibody (Cat#6997, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human hepatocarcinoma using AKR1C4 antibody (Cat#6997, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
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