• Primary Antibody
  • Secondary Antibodies
  • Lentiviral Particles
  • Stable Cell Lines
  • Lysates
  • Reagents
  • Other Products

KO-Validated Phospho-PRKAA2 (S345) Recombinant Rabbit mAb

#71187

Click to copy product information
Recombinant Antibody
Knockout Validated
Western blotting analysis using phospho-PRKAA2 (S345) antibody (Cat#71187). Phospho-PRKAA2 (S345) expression in wild type (WT) and PRKAA2 knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with phospho-PRKAA2 (S345) antibody (Cat#71187, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Phospho-PRKAA2 (S345) expression in HeLa cells using Phospho-PRKAA2 (S345) antibody (Cat#71187, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Phospho-PRKAA2 (S345).
Please select the specifications of the product.
Specification:
Unit price:
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    PRKAA2
  • Catalog:
    71187
  • Application:
    WB, FCM
  • Species Reactivity:
    H
Information
Product Name KO-Validated Phospho-PRKAA2 (S345) Recombinant Rabbit mAb
Aliases PRKAA2; Protein Kinase AMP-Activated Catalytic Subunit Alpha 2; AMPK; 5'-AMP-Activated Protein Kinase Catalytic Subunit Alpha-2; Protein Kinase, AMP-Activated, Alpha 2 Catalytic Subunit; Hydroxymethylglutaryl-CoA Reductase Kinase; Acetyl-CoA Carboxylase Kinase; AMPK Subunit Alpha-2; ACACA Kinase; HMGCR Kinase; EC 2.7.11.1; AMPKa2; AMPK2; PRKAA; 5'-AMP-Activated Protein Kinase, Catalytic Alpha-2 Chain; AMPK-Alpha-2 Chain; AMPK Alpha; EC 2.7.11.27; EC 2.7.11.31; EC 2.7.11 48; AMPKA2
Background

Gene Name: PRKAA2

NCBI Gene Entry: 5563

UniProt Entry: P54646

Application Information

Molecular Weight: Predicted, 62 kDa; observed, 64 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB2285

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human Phospho-PRKAA2 (S345)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using phospho-PRKAA2 (S345) antibody (Cat#71187). Phospho-PRKAA2 (S345) expression in wild type (WT) and PRKAA2 knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with phospho-PRKAA2 (S345) antibody (Cat#71187, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of Phospho-PRKAA2 (S345) expression in HeLa cells using Phospho-PRKAA2 (S345) antibody (Cat#71187, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Phospho-PRKAA2 (S345).
Western blotting analysis using phospho-PRKAA2 (S345) antibody (Cat#71187). Phospho-PRKAA2 (S345) expression in wild type (WT) and PRKAA2 knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with phospho-PRKAA2 (S345) antibody (Cat#71187, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Phospho-PRKAA2 (S345) expression in HeLa cells using Phospho-PRKAA2 (S345) antibody (Cat#71187, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Phospho-PRKAA2 (S345).
  • Phone Number

    +1 (301) 545-2395

  • Email Address

    support@genuinbiotech.com

    sales@genuinbiotech.com

  • Office Hours

    8:30 AM - 5:30 PM EST Mon-Fri

微信公众号

Copyright © 2025 Hefei GenuIN Biotech Co., Ltd.

All Rights Reserved.