Western blotting analysis using phospho-Histone H3 (T3) antibody (Cat#71194). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-Histone H3 (T3) antibody (Cat#71194, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using phospho-histone H3 (T3) antibody (Cat#71194). Phospho-histone H3 (T3) expression in wild type (WT) and H3-3A knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with phospho-histone H3 (T3) antibody (Cat#71194, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with Phospho-Histone H3 (T3) antibody (Cat#71194, 1:1,000). Nuclei were stained blue with DAPI; Phospho-Histone H3 (T3) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
