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KO-Validated ALDH1A3 Mouse mAb

#71254

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Knockout Validated
Western blotting analysis using ALDH1A3 antibody (Cat#71254). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ALDH1A3 antibody (Cat#71254, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using ALDH1A3 antibody (Cat#71254). ALDH1A3 expression in wild-type (WT) and ALDH1A3 knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ALDH1A3 antibody (Cat#71254, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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  • Gene Symbol:
    ALDH1A3
  • Catalog:
    71254
  • Application:
    WB
  • Species Reactivity:
    H
Information
Product Name KO-Validated ALDH1A3 Mouse mAb
Aliases ALDH1A3; Aldehyde Dehydrogenase 1 Family Member A3; Retinaldehyde Dehydrogenase 3; RALDH3; ALDH6; Aldehyde Dehydrogenase 6; Aldehyde Dehydrogenase 1 Family, Member A3; Aldehyde Dehydrogenase Family 1 Member A3; Acetaldehyde Dehydrogenase 6; EC 1.2.1.36; EC 1.2.1; ALDH1A6; RALDH-3; RalDH3; MCOP8
Background

Gene Name: ALDH1A3

NCBI Gene Entry: 220

UniProt Entry: P47895

Application Information

Molecular Weight: Predicted, 56 kDa; observed, 56 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB3805

Species Reactivity: Human

Applications Tested: Western blotting (WB)

Immunogen Recombinant protein of human ALDH1A3
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Note This product is for research use only.
Data
  • Western blotting analysis using ALDH1A3 antibody (Cat#71254). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ALDH1A3 antibody (Cat#71254, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using ALDH1A3 antibody (Cat#71254). ALDH1A3 expression in wild-type (WT) and ALDH1A3 knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ALDH1A3 antibody (Cat#71254, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using ALDH1A3 antibody (Cat#71254). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ALDH1A3 antibody (Cat#71254, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using ALDH1A3 antibody (Cat#71254). ALDH1A3 expression in wild-type (WT) and ALDH1A3 knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ALDH1A3 antibody (Cat#71254, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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