Western blotting analysis using folate hydrolase 1 antibody (Cat#71263). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with folate hydrolase 1 antibody (Cat#71263, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using folate hydrolase 1 antibody (Cat#71263). Folate hydrolase 1 expression in wild-type (WT) and folate hydrolase 1 (FOLH1) knockout (KO) HSHC cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with folate hydrolase 1 antibody (Cat#71263, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Folate hydrolase 1 expression in HAP-1 cells using Folate hydrolase 1 antibody (Cat#71263, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Folate hydrolase 1.
