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Home>Product>Primary Antibody

KO-Validated ATP1A1 Recombinant Rabbit mAb

#71282

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Recombinant Antibody
Knockout Validated
Western blotting analysis using ATP1A1 antibody (Cat#71282). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATP1A1 antibody (Cat#71282, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using ATP1A1 antibody (Cat#71282). ATP1A1 expression in wild-type (WT) and ATP1A1 knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ATP1A1 antibody (Cat#71282, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using ATP1A1 antibody (Cat#71282, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
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  • Gene Symbol：
    ATP1A1
  • Catalog：
    71282
  • Application：
    WB, IHC-P
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KO-Validated ATP1A1 Recombinant Rabbit mAb
Aliases ATP1A1; ATPase Na+/K+ Transporting Subunit Alpha 1; Sodium/Potassium-Transporting ATPase Subunit Alpha-1; Sodium Pump Subunit Alpha-1; Sodium-Potassium ATPase Catalytic Subunit Alpha-1; ATPase, Na+/K+ Transporting, Alpha 1 Polypeptide; Na(+)/K(+) ATPase Alpha-1 Subunit; Na,K-ATPase Catalytic Subunit Alpha-A Protein; Sodium-Potassium-ATPase, Alpha 1 Polypeptide; Na, K-ATPase, Alpha-A Catalytic Polypeptide; Na,K-ATPase Alpha-1 Subunit; Na+/K+ ATPase 1; EC 7.2.2.13; EC 3.6.3.9; HOMGSMR2; EC 3.6.3; CMT2DD
Background

Gene Name: ATP1A1

NCBI Gene Entry: 476

UniProt Entry: P05023

Application Information

Molecular Weight: Predicted, 113 kDa; observed, 85,110 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB6185

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), immunohistochemistry-paraffin (IHC-P)
Immunogen A synthesized peptide derived from human Sodium Potassium ATPase
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,000-1:10,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using ATP1A1 antibody (Cat#71282). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATP1A1 antibody (Cat#71282, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using ATP1A1 antibody (Cat#71282). ATP1A1 expression in wild-type (WT) and ATP1A1 knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ATP1A1 antibody (Cat#71282, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using ATP1A1 antibody (Cat#71282, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Please select the specifications of the product.
Specification：
Unit price：
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ATP1A1
  • Catalog：
    71282
  • Application：
    WB, IHC-P
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using ATP1A1 antibody (Cat#71282). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATP1A1 antibody (Cat#71282, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using ATP1A1 antibody (Cat#71282). ATP1A1 expression in wild-type (WT) and ATP1A1 knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ATP1A1 antibody (Cat#71282, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using ATP1A1 antibody (Cat#71282, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
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