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KO-Validated CDKN2A Mouse mAb

#71292

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Knockout Validated
Western blotting analysis using CDKN2A antibody (Cat#71292). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CDKN2A antibody (Cat#71292, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using CDKN2A antibody (Cat#71292). CDKN2A expression in wild-type (WT) and CDKN2A knockout (KO) 293T cells with 20 μg of total cell lysates. GAPDH serves as a loading control. The blot was incubated with CDKN2A antibody (Cat#71292, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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  • Gene Symbol:
    CDKN2A
  • Catalog:
    71292
  • Application:
    WB
  • Species Reactivity:
    H
Information
Product Name KO-Validated CDKN2A Mouse mAb
Aliases CDKN2A; Cyclin Dependent Kinase Inhibitor 2A; P14ARF; CDK4I; MTS1; ARF; Cyclin-Dependent Kinase 4 Inhibitor A; P16-INK4A; P16INK4A; CDKN2; CMM2; INK4; P16; P19; P14; MLM; Cyclin-Dependent Kinase Inhibitor 2A (Melanoma, P16, Inhibits CDK4); Cyclin-Dependent Kinase Inhibitor 2A; Multiple Tumour Suppressor 1; Multiple Tumor Suppressor 1; CDKN2A/ARF Intron 2 LncRNA; Alternative Reading Frame; Inhibitor Of Cdk4 A; P19ARF; INK4A; MTS-1; CAI2; Cell Cycle Negative Regulator Beta; P19 Alternate Open Reading Frame; P14 Alternate Open Reading Frame; CDK4 Inhibitor P16-INK4; Tumor Suppressor ARF; P16-INK4a; P16INK4a; P16-INK4; P16INK4; P19Arf; INK4a; TP16
Background

Gene Name: CDKN2A

NCBI Gene Entry: 1029

UniProt Entry: P42771

Application Information

Molecular Weight: Predicted, 17 kDa; observed, 18 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 26GB1015

Species Reactivity: Human

Applications Tested: Western blotting (WB)

Immunogen Recombinant protein of human CDKN2A
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using CDKN2A antibody (Cat#71292). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CDKN2A antibody (Cat#71292, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using CDKN2A antibody (Cat#71292). CDKN2A expression in wild-type (WT) and CDKN2A knockout (KO) 293T cells with 20 μg of total cell lysates. GAPDH serves as a loading control. The blot was incubated with CDKN2A antibody (Cat#71292, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using CDKN2A antibody (Cat#71292). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CDKN2A antibody (Cat#71292, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using CDKN2A antibody (Cat#71292). CDKN2A expression in wild-type (WT) and CDKN2A knockout (KO) 293T cells with 20 μg of total cell lysates. GAPDH serves as a loading control. The blot was incubated with CDKN2A antibody (Cat#71292, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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