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KO-Validated SERPINA1 Mouse mAb

#71295

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Knockout Validated
Western blotting analysis using SERPINA1 antibody (Cat#71295). SERPINA1 expression in wild-type (WT) and SERPINA1 knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with SERPINA1 antibody (Cat#71295, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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  • Gene Symbol:
    SERPINA1
  • Catalog:
    71295
  • Application:
    WB
  • Species Reactivity:
    H
Information
Product Name KO-Validated SERPINA1 Mouse mAb
Aliases SERPINA1; Serpin Family A Member 1; Alpha-1-Antitrypsin; AAT; Alpha1AT; A1AT; A1A; PI1; PI; Serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1; Alpha-1 Proteinase Inhibitor; Alpha-1 Protease Inhibitor; Alpha-1-Antiproteinase; Alpha-1 Antitrypsin; Anti-Elastase; Serpin A1; Serine (Or Cysteine) Proteinase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1; Serpin Peptidase Inhibitor Clade A (Alpha-1antiproteinase, Antitrypsin) Member 1; Serine (Or Cysteine) Proteinase Inhibitor, Clade A, Member 1; Protease Inhibitor 1 (Anti-Elastase), Alpha-1-Antitrypsin; Serpin Peptidase Inhibitor Clade A Member 1; Epididymis Secretory Sperm Binding Protein; Protease Inhibitor 1; PRO2275; NNIF
Background

Gene Name: SERPINA1

NCBI Gene Entry: 5265

UniProt Entry: P01009

Application Information

Molecular Weight: Predicted, 47 kDa; observed, 55 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 26GB1570

Species Reactivity: Human

Applications Tested: Western blotting (WB)

Immunogen Recombinant protein of human SERPINA1
Isotype Mouse IgG2b
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.03% Proclin 300.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Note This product is for research use only.
Data
  • Western blotting analysis using SERPINA1 antibody (Cat#71295). SERPINA1 expression in wild-type (WT) and SERPINA1 knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with SERPINA1 antibody (Cat#71295, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using SERPINA1 antibody (Cat#71295). SERPINA1 expression in wild-type (WT) and SERPINA1 knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with SERPINA1 antibody (Cat#71295, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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