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KO-Validated RELA Mouse mAb

#71308

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Knockout Validated
Western blotting analysis using RELA antibody (Cat#71308). RELA expression in wild-type (WT) and RELA knockout (KO) HeLa cells with 20 μg of total cell lysates. GAPDH serves as a loading control. The blot was incubated with RELA antibody (Cat#71308, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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  • Gene Symbol:
    RELA
  • Catalog:
    71308
  • Application:
    WB
  • Species Reactivity:
    H
Information
Product Name KO-Validated RELA Mouse mAb
Aliases RELA; RELA Proto-Oncogene, NF-KB Subunit; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 3; NFKB3; P65; V-Rel Avian Reticuloendotheliosis Viral Oncogene Homolog A; Nuclear Factor NF-Kappa-B P65 Subunit; Transcription Factor P65; NF-Kappa-B Transcription Factor P65; NF-Kappa-B P65delta3; AIF3BL3; CMCU
Background

Gene Name: RELA

NCBI Gene Entry: 5970

UniProt Entry: Q04206

Application Information

Molecular Weight: Predicted, 60 kDa; observed, 68 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 26GB2120

Species Reactivity: Human

Applications Tested: Western blotting (WB)

Immunogen Recombinant protein of human RELA
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using RELA antibody (Cat#71308). RELA expression in wild-type (WT) and RELA knockout (KO) HeLa cells with 20 μg of total cell lysates. GAPDH serves as a loading control. The blot was incubated with RELA antibody (Cat#71308, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using RELA antibody (Cat#71308). RELA expression in wild-type (WT) and RELA knockout (KO) HeLa cells with 20 μg of total cell lysates. GAPDH serves as a loading control. The blot was incubated with RELA antibody (Cat#71308, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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