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Erb-B2 Receptor Tyrosine Kinase 4 Recombinant Rabbit mAb

#8611

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Recombinant Antibody
Western blotting analysis using Erb-b2 receptor tyrosine kinase 4 antibody (Cat#8611). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Erb-b2 receptor tyrosine kinase 4 antibody (Cat#8611, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with Erb-b2 receptor tyrosine kinase 4 antibody (Cat#8611, 1:1,000). Nuclei were stained blue with DAPI; Erb-b2 receptor tyrosine kinase 4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    ERBB4
  • Catalog:
    8611
  • Application:
    WB, ICC
  • Species Reactivity:
    H
Information
Product Name Erb-B2 Receptor Tyrosine Kinase 4 Recombinant Rabbit mAb
Aliases Erb-B2 Receptor Tyrosine Kinase 4; HER4;ALS19;V-Erb-B2 Avian Erythroblastic Leukemia Viral Oncogene Homolog; Tyrosine Kinase-Type Cell Surface Receptor HER4; Human Epidermal Growth Factor Receptor; Receptor Tyrosine-Protein Kinase ErbB-4; Proto-Oncogene-Like Protein C-ErbB-4; EC 2.7.10.1; P180erbB4; V-Erb-A Avian Erythroblastic Leukemia Viral Oncogene Homolog-Like; Avian Erythroblastic Leukemia Viral (V-Erb-B2) Oncogene Homolog; V-Erb-A Erythroblastic Leukemia Viral Oncogene Homolog; EC 2.7.10
Background

Gene Name: ERBB4

NCBI Gene Entry: 2066

UniProt Entry: Q15303

Application Information

Molecular Weight: Predicted, 147 kDa; observed, 160 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB1245

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human ErbB4 (HER4)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Erb-b2 receptor tyrosine kinase 4 antibody (Cat#8611). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Erb-b2 receptor tyrosine kinase 4 antibody (Cat#8611, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HAP-1 cells with Erb-b2 receptor tyrosine kinase 4 antibody (Cat#8611, 1:1,000). Nuclei were stained blue with DAPI; Erb-b2 receptor tyrosine kinase 4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Western blotting analysis using Erb-b2 receptor tyrosine kinase 4 antibody (Cat#8611). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Erb-b2 receptor tyrosine kinase 4 antibody (Cat#8611, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with Erb-b2 receptor tyrosine kinase 4 antibody (Cat#8611, 1:1,000). Nuclei were stained blue with DAPI; Erb-b2 receptor tyrosine kinase 4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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