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Home>Product>Primary Antibody

Chromobox 4 Recombinant Rabbit mAb

#9184

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Recombinant Antibody
Western blotting analysis using Chromobox 4 antibody (Cat#9184). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Chromobox 4 antibody (Cat#9184, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Chromobox 4 antibody (Cat#9184, 1:1,000). Nuclei were stained blue with DAPI; Chromobox 4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Chromobox 4 expression in HepG2 cells using Chromobox 4 antibody (Cat#9184, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Chromobox 4.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CBX4
  • Catalog：
    9184
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Chromobox 4 Recombinant Rabbit mAb
Aliases Chromobox 4; NBP16; PC2; E3 SUMO-Protein Ligase CBX4; Chromobox Protein Homolog 4; NS5ATP1-Binding Protein 16; Polycomb 2 Homolog; HPC2; Chromobox Homolog 4 (Pc Class Homolog, Drosophila); Chromobox Homolog 4 (Drosophila Pc Class); Pc Class 2 Homolog (Drosophila); Chromobox-Like Protein 4; Chromobox Homolog 4; Pc Class 2 Homolog; EC 2.3.2.-; HPc2; Pc2
Background

Gene Name: CBX4

NCBI Gene Entry: 8535

UniProt Entry: O00257

Application Information

Molecular Weight: Predicted, 61 kDa; observed, 50 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB1905

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human CBX4
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Chromobox 4 antibody (Cat#9184). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Chromobox 4 antibody (Cat#9184, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with Chromobox 4 antibody (Cat#9184, 1:1,000). Nuclei were stained blue with DAPI; Chromobox 4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Chromobox 4 expression in HepG2 cells using Chromobox 4 antibody (Cat#9184, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Chromobox 4.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CBX4
  • Catalog：
    9184
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using Chromobox 4 antibody (Cat#9184). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Chromobox 4 antibody (Cat#9184, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Chromobox 4 antibody (Cat#9184, 1:1,000). Nuclei were stained blue with DAPI; Chromobox 4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Chromobox 4 expression in HepG2 cells using Chromobox 4 antibody (Cat#9184, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Chromobox 4.
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