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Home>Product>Primary Antibody

KD-Validated ERBB2 Recombinant Rabbit mAb

#H1113

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Western blotting analysis using erb-b2 receptor tyrosine kinase 2 antibody (Cat#H1113). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with erb-b2 receptor tyrosine kinase 2 antibody (Cat#H1113, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using erb-b2 receptor tyrosine kinase 2 antibody (Cat#H1113). Erb-b2 receptor tyrosine kinase 2 expression in wild type (WT) and erb-b2 receptor tyrosine kinase 2 (ERBB2) shRNA knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with erb-b2 receptor tyrosine kinase 2 antibody (Cat#H1113, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining shows negative staining in paraffin-embedded human breast. Erb-b2 receptor tyrosine kinase 2 antibody (Cat#H1113) was used at 1:1,000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.Counterstained with hematoxylin.Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human breast cancer. Erb-b2 receptor tyrosine kinase 2 antibody (Cat#H1113) was used at 1:1,000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.Counterstained with hematoxylin.Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human breast cancer. Erb-b2 receptor tyrosine kinase 2 antibody (Cat#H1113) was used at 1:1,000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.Counterstained with hematoxylin.Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Flow cytometric analysis of Erb-b2 receptor tyrosine kinase 2 expression in A549 cells using Erb-b2 receptor tyrosine kinase 2 antibody (Cat#H1113, 1:2,000). Green, isotype control; red, Erb-b2 receptor tyrosine kinase 2.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ERBB2
  • Catalog：
    H1113
  • Application：
    WB, IHC-P, FCM
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated ERBB2 Recombinant Rabbit mAb
Aliases ERBB2; Erb-B2 Receptor Tyrosine Kinase 2; HER2; NEU 2; C-ERB-2; C-ERB2; MLN-19; HER-2; CD340; NGL; V-Erb-B2 Avian Erythroblastic Leukemia Viral Oncogene Homolog 2; Tyrosine Kinase-Type Cell Surface Receptor HER2; Neuro/Glioblastoma Derived Oncogene Homolog; Human Epidermal Growth Factor Receptor 2; Receptor Tyrosine-Protein Kinase ErbB-2; Metastatic Lymph Node Gene 19 Protein; Proto-Oncogene C-ErbB-2; Proto-Oncogene Neu; P185(ErbB2); EC 2.7.10.1; MLN 19; V-Erb-B2 Avian Erythroblastic Leukemia Viral Oncogene Homolog 2 (Neuro/Glioblastoma Derived Oncogene Homolog) 2; V-Erb-B2 Erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/Glioblastoma Derived Oncogene Homolog; V-Erb-B2 Avian Erythroblastic Leukemia Viral Oncoprotein 2; Neuroblastoma/Glioblastoma Derived Oncogene Homolog; Metastatic Lymph Node Gene 19; C-Erb B2/Neu Protein; CD340 Antigen; P185(ERBB2); HER-2/Neu; Herstatin; P185erbB2; EC 2.7.10; VSCN2; MLN19; TKR1
Background

Gene Name: ERBB2

NCBI Gene Entry: 2064

UniProt Entry: P04626

Application Information

Molecular Weight: Predicted, 138 kDa; observed, 185 kDa

Clonality: Recombinant Monoclonal Antibody

Clone ID: SDT-069-57

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunohistochemistry-paraffin (IHC-P), flow cytometry (FCM)
Immunogen A synthesized peptide derived from human ErbB2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 40% glycerol, 0.05% BSA and 0.03% Proclin 300.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunohistochemistry-Paraffin (IHC-P): 1:1,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using erb-b2 receptor tyrosine kinase 2 antibody (Cat#H1113). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with erb-b2 receptor tyrosine kinase 2 antibody (Cat#H1113, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using erb-b2 receptor tyrosine kinase 2 antibody (Cat#H1113). Erb-b2 receptor tyrosine kinase 2 expression in wild type (WT) and erb-b2 receptor tyrosine kinase 2 (ERBB2) shRNA knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with erb-b2 receptor tyrosine kinase 2 antibody (Cat#H1113, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining shows negative staining in paraffin-embedded human breast. Erb-b2 receptor tyrosine kinase 2 antibody (Cat#H1113) was used at 1:1,000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.Counterstained with hematoxylin.Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Immunocytochemical staining shows positive staining in paraffin-embedded human breast cancer. Erb-b2 receptor tyrosine kinase 2 antibody (Cat#H1113) was used at 1:1,000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.Counterstained with hematoxylin.Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Immunocytochemical staining shows positive staining in paraffin-embedded human breast cancer. Erb-b2 receptor tyrosine kinase 2 antibody (Cat#H1113) was used at 1:1,000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.Counterstained with hematoxylin.Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Flow cytometric analysis of Erb-b2 receptor tyrosine kinase 2 expression in A549 cells using Erb-b2 receptor tyrosine kinase 2 antibody (Cat#H1113, 1:2,000). Green, isotype control; red, Erb-b2 receptor tyrosine kinase 2.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ERBB2
  • Catalog：
    H1113
  • Application：
    WB, IHC-P, FCM
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using erb-b2 receptor tyrosine kinase 2 antibody (Cat#H1113). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with erb-b2 receptor tyrosine kinase 2 antibody (Cat#H1113, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using erb-b2 receptor tyrosine kinase 2 antibody (Cat#H1113). Erb-b2 receptor tyrosine kinase 2 expression in wild type (WT) and erb-b2 receptor tyrosine kinase 2 (ERBB2) shRNA knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with erb-b2 receptor tyrosine kinase 2 antibody (Cat#H1113, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining shows negative staining in paraffin-embedded human breast. Erb-b2 receptor tyrosine kinase 2 antibody (Cat#H1113) was used at 1:1,000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.Counterstained with hematoxylin.Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human breast cancer. Erb-b2 receptor tyrosine kinase 2 antibody (Cat#H1113) was used at 1:1,000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.Counterstained with hematoxylin.Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human breast cancer. Erb-b2 receptor tyrosine kinase 2 antibody (Cat#H1113) was used at 1:1,000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.Counterstained with hematoxylin.Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Flow cytometric analysis of Erb-b2 receptor tyrosine kinase 2 expression in A549 cells using Erb-b2 receptor tyrosine kinase 2 antibody (Cat#H1113, 1:2,000). Green, isotype control; red, Erb-b2 receptor tyrosine kinase 2.
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