KD-Validated CD38 Recombinant Rabbit mAb - Product

KD-Validated CD38 Recombinant Rabbit mAb

#H1117

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Western blotting analysis using CD38 antibody (Cat#H1117). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CD38 antibody (Cat#H1117, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226).

Western blotting analysis using CD38 antibody (Cat#H1117). CD38 expression in wild type (WT) and CD38 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with CD38 antibody (Cat#H1117, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Immunocytochemical staining shows positive staining in paraffin-embedded human spleen. CD38 antibody (Cat#H1117) was used at 1:1,000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.

Immunocytochemical staining shows positive staining in paraffin-embedded human tonsil. CD38 antibody (Cat#H1117) was used at 1:1,000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.

Flow cytometric analysis of CD38 expression in Raji cells using CD38 antibody (Cat#H1117, 1:2,000). Green, isotype control; red, CD38.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

CD38
Catalog：

H1117
Application：

WB, IHC-P, FCM
Species Reactivity：

H, R

Information

Data

Information

Product Name	KD-Validated CD38 Recombinant Rabbit mAb
Aliases	CD38; CD38 Molecule; ADP-Ribosyl Cyclase 1; CADPR1; ADP-Ribosyl Cyclase/Cyclic ADP-Ribose Hydrolase 1; 2'-Phospho-Cyclic-ADP-Ribose Transferase; 2'-Phospho-ADP-Ribosyl Cyclase; Cyclic ADP-Ribose Hydrolase 1; NAD(+) Nucleosidase; CD38 Antigen (P45); ADPRC 1; 2'-Phospho-ADP-Ribosyl Cyclase/2'-Phospho-Cyclic-ADP-Ribose Transferase; Ecto-Nicotinamide Adenine Dinucleotide Glycohydrolase; Cluster Of Differentiation 38; CADPR Hydrolase 1; CD38 Antigen; EC 2.4.99.20; EC 3.2.2.-; EC 3.2.2.6; ADPRC1; T10
Background	Gene Name: CD38 NCBI Gene Entry: 952 UniProt Entry: P28907
Application Information	Molecular Weight: Predicted, 34 kDa; observed, 45 kDa Clonality: Recombinant Monoclonal Antibody Clone ID: SDT-031-45-2 Species Reactivity: Human, rat Applications Tested: Western blotting (WB), immunohistochemistry-paraffin (IHC-P), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human CD38
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 40% glycerol, 0.05% BSA and 0.03% Proclin 300.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:500-1:2,500 Immunohistochemistry-Paraffin (IHC-P): 1:1,000 Flow Cytometry (FCM): 1:200-1:2,000
Note	This product is for research use only.

Data

Western blotting analysis using CD38 antibody (Cat#H1117). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CD38 antibody (Cat#H1117, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226).
Western blotting analysis using CD38 antibody (Cat#H1117). CD38 expression in wild type (WT) and CD38 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with CD38 antibody (Cat#H1117, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining shows positive staining in paraffin-embedded human spleen. CD38 antibody (Cat#H1117) was used at 1:1,000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human tonsil. CD38 antibody (Cat#H1117) was used at 1:1,000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Flow cytometric analysis of CD38 expression in Raji cells using CD38 antibody (Cat#H1117, 1:2,000). Green, isotype control; red, CD38.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

CD38
Catalog：

H1117
Application：

WB, IHC-P, FCM
Species Reactivity：

H, R

Datasheet

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