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Home>Product>Primary Antibody

MGMT Recombinant Rabbit mAb

#H1143

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Western blotting analysis using O-6-methylguanine-DNA methyltransferase antibody (Cat#H1143). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with O-6-methylguanine-DNA methyltransferase antibody (Cat#H1143, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining shows positive staining in paraffin-embedded human diffuse large B-cell lymphoma. MGMT antibody (Cat#H1143) was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human stomach. MGMT antibody (Cat#H1143) was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human testis. MGMT antibody (Cat#H1143) was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Flow cytometric analysis of O-6-methylguanine-DNA methyltransferase expression in HepG2 cells using O-6-methylguanine-DNA methyltransferase antibody (Cat#H1143, 1:2,000). Green, isotype control; red, O-6-methylguanine-DNA methyltransferase.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    MGMT
  • Catalog：
    H1143
  • Application：
    WB, IHC-P, FCM
  • Species Reactivity：
    H

Information

Data

Information
Product Name MGMT Recombinant Rabbit mAb
Aliases MGMT; O-6-Methylguanine-DNA Methyltransferase; Methylated-DNA--Protein-Cysteine Methyltransferase; 6-O-Methylguanine-DNA Methyltransferase; O-6-Methylguanine-DNA-Alkyltransferase; EC 2.1.1.63; O6-Methylguanine-DNA Methyltransferase; Methylguanine-DNA Methyltransferase
Background

Gene Name: MGMT

NCBI Gene Entry: 4255

UniProt Entry: P16455

Application Information

Molecular Weight: Predicted, 22 kDa; observed, 21 kDa

Clonality: Recombinant Monoclonal Antibody

Clone ID: SDT-522-71

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunohistochemistry-paraffin (IHC-P), flow cytometry (FCM)
Immunogen A synthesized peptide derived from human MGMT
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 40% glycerol, 0.05% BSA and 0.03% Proclin 300.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Immunohistochemistry-Paraffin (IHC-P): 1:100
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using O-6-methylguanine-DNA methyltransferase antibody (Cat#H1143). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with O-6-methylguanine-DNA methyltransferase antibody (Cat#H1143, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining shows positive staining in paraffin-embedded human diffuse large B-cell lymphoma. MGMT antibody (Cat#H1143) was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Immunocytochemical staining shows positive staining in paraffin-embedded human stomach. MGMT antibody (Cat#H1143) was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Immunocytochemical staining shows positive staining in paraffin-embedded human testis. MGMT antibody (Cat#H1143) was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Flow cytometric analysis of O-6-methylguanine-DNA methyltransferase expression in HepG2 cells using O-6-methylguanine-DNA methyltransferase antibody (Cat#H1143, 1:2,000). Green, isotype control; red, O-6-methylguanine-DNA methyltransferase.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    MGMT
  • Catalog：
    H1143
  • Application：
    WB, IHC-P, FCM
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using O-6-methylguanine-DNA methyltransferase antibody (Cat#H1143). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with O-6-methylguanine-DNA methyltransferase antibody (Cat#H1143, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining shows positive staining in paraffin-embedded human diffuse large B-cell lymphoma. MGMT antibody (Cat#H1143) was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human stomach. MGMT antibody (Cat#H1143) was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human testis. MGMT antibody (Cat#H1143) was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Flow cytometric analysis of O-6-methylguanine-DNA methyltransferase expression in HepG2 cells using O-6-methylguanine-DNA methyltransferase antibody (Cat#H1143, 1:2,000). Green, isotype control; red, O-6-methylguanine-DNA methyltransferase.
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