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Home>Product>Primary Antibody

PIK3R1 Rabbit mAb

#R0004

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Western blotting analysis using anti-PI3 Kinase p85α antibody (Cat#R0004). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PI3 Kinase p85α antibody (Cat#R0004, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with anti-PI3 Kinase p85α antibody (Cat#R0004, 1:1,000). Nuclei were stained blue with DAPI; PI3 Kinase p85α was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Flow cytometric analysis of PI3 Kinase p85α expression in C2C12 cells using anti-PI3 Kinase p85α antibody (Cat#R0004, 1:2,000). Green, isotype control; red, PI3 Kinase p85α.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PIK3R1
  • Catalog：
    R0004
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name PIK3R1 Rabbit mAb
Aliases PIK3R1; Phosphoinositide-3-Kinase Regulatory Subunit 1; GRB1; P85-ALPHA; P85; Phosphatidylinositol 3-Kinase 85 KDa Regulatory Subunit Alpha; Phosphoinositide-3-Kinase, Regulatory Subunit 1 (Alpha); Phosphatidylinositol 3-Kinase Regulatory Subunit Alpha; Phosphoinositide-3-Kinase Regulatory Subunit Alpha; PtdIns-3-Kinase Regulatory Subunit Alpha; Growth Factor Receptor Bound 1; PI3K Regulatory Subunit Alpha; PI3-Kinase Subunit P85-Alpha; PI3 Kinase-Associated P85; P85alpha; Phosphatidylinositol 3-Kinase, Regulatory Subunit, Polypeptide 1 (P85 Alpha); Phosphatidylinositol 3-Kinase-Associated P-85 Alpha; PtdIns-3-Kinase Regulatory Subunit P85-Alpha; PI3-Kinase Regulatory Subunit Alpha; P85ALPHA; IMD36; AGM7
Background

Gene Name: PIK3R1

NCBI Gene Entry: 5295

UniProt Entry: P27986

Application Information

Molecular Weight: Predicted, 84 kDa; observed, 74 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: S-328-24

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human PIK3R1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 40% glycerol, 0.05% BSA and 0.03% Proclin 300.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using anti-PI3 Kinase p85α antibody (Cat#R0004). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PI3 Kinase p85α antibody (Cat#R0004, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of C2C12 cells with anti-PI3 Kinase p85α antibody (Cat#R0004, 1:1,000). Nuclei were stained blue with DAPI; PI3 Kinase p85α was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Flow cytometric analysis of PI3 Kinase p85α expression in C2C12 cells using anti-PI3 Kinase p85α antibody (Cat#R0004, 1:2,000). Green, isotype control; red, PI3 Kinase p85α.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PIK3R1
  • Catalog：
    R0004
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using anti-PI3 Kinase p85α antibody (Cat#R0004). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PI3 Kinase p85α antibody (Cat#R0004, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with anti-PI3 Kinase p85α antibody (Cat#R0004, 1:1,000). Nuclei were stained blue with DAPI; PI3 Kinase p85α was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Flow cytometric analysis of PI3 Kinase p85α expression in C2C12 cells using anti-PI3 Kinase p85α antibody (Cat#R0004, 1:2,000). Green, isotype control; red, PI3 Kinase p85α.
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