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Home>Product>Primary Antibody

KD-Validated MEK1/2 Rabbit mAb

#R0020

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Knockdown Validated
Western blotting analysis using anti-MEK1/2 antibody (Cat#R0020). MEK1/2 expression in wild-type (WT) and MAP2K1 shRNA knockdown (KD) HeLa cells with 10 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-MEK1/2 antibody (Cat#R0020, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-MEK1/2 antibody (Cat#R0020). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MEK1/2 antibody (Cat#R0020, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with anti-MEK1/2 antibody (Cat#R0020, 1:1,000). Nuclei were stained blue with DAPI; MEK1/2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Flow cytometric analysis of MEK1/2 expression in HepG2 cells using anti-MEK1/2 antibody (Cat#R0020, 1:2,000). Green, isotype control; red, MEK1/2.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    MP2K1/MAP2K2
  • Catalog：
    R0020
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated MEK1/2 Rabbit mAb
Aliases MP2K1; Mitogen-Activated Protein Kinase Kinase 1; MEK1; Dual Specificity Mitogen-Activated Protein Kinase Kinase 1; MAPK/ERK Kinase 1; MAPKK1; PRKMK1; MKK1; ERK Activator Kinase 1; MAP Kinase Kinase 1; EC 2.7.12.2; MAPKK 1; MEK 1; Protein Kinase, Mitogen-Activated, Kinase 1 (MAP Kinase Kinase 1); CFC3; MEL; MAP2K2; Mitogen-Activated Protein Kinase Kinase 2; MEK2; MAP Kinase Kinase 2; PRKMK2; MKK2; Dual Specificity Mitogen-Activated Protein Kinase Kinase 2; ERK Activator Kinase 2; MAPK/ERK Kinase 2; EC 2.7.12.2; Mitogen-Activated Protein Kinase Kinase 2, P45; MAPKK 2; MAPKK2; MEK 2; CFC4
Background

Gene Name: MP2K1/MAP2K2

NCBI Gene Entry: 5604/5605

UniProt Entry: Q02750/P36507

Application Information

Molecular Weight: Predicted, 43/44 kDa; observed, 45 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: S-834-101

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human MP2K1/MAP2K2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 40% glycerol, 0.05% BSA and 0.03% Proclin 300.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1::200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using anti-MEK1/2 antibody (Cat#R0020). MEK1/2 expression in wild-type (WT) and MAP2K1 shRNA knockdown (KD) HeLa cells with 10 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-MEK1/2 antibody (Cat#R0020, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-MEK1/2 antibody (Cat#R0020). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MEK1/2 antibody (Cat#R0020, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with anti-MEK1/2 antibody (Cat#R0020, 1:1,000). Nuclei were stained blue with DAPI; MEK1/2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Flow cytometric analysis of MEK1/2 expression in HepG2 cells using anti-MEK1/2 antibody (Cat#R0020, 1:2,000). Green, isotype control; red, MEK1/2.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    MP2K1/MAP2K2
  • Catalog：
    R0020
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using anti-MEK1/2 antibody (Cat#R0020). MEK1/2 expression in wild-type (WT) and MAP2K1 shRNA knockdown (KD) HeLa cells with 10 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-MEK1/2 antibody (Cat#R0020, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-MEK1/2 antibody (Cat#R0020). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MEK1/2 antibody (Cat#R0020, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with anti-MEK1/2 antibody (Cat#R0020, 1:1,000). Nuclei were stained blue with DAPI; MEK1/2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Flow cytometric analysis of MEK1/2 expression in HepG2 cells using anti-MEK1/2 antibody (Cat#R0020, 1:2,000). Green, isotype control; red, MEK1/2.
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