GFP Tag Mouse mAb - Product - Hefei GenuIN Biotech Co., Ltd.

GFP Tag Mouse mAb

#T028

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Western blotting analysis using GFP-tag antibody (Cat#T028). Recombinant GFP (100 ng) expressed by E. coli were loaded and separated by SDS-PAGE. The blot was incubated with GFP-tag antibody (Cat#T028, 1:20,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Immunocytochemical staining of 293T cells transfected with GFP protein using GFP antibody (Cat#T028, 1:1,000). Nuclei were stained blue with DAPI; GFP was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.

Flow cytometric analysis of 293T cells transfected with GFP protein using GFP antibody (Cat#T028, 1:2,000). Green, isotype control; red, GFP.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$69.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：
Catalog：

T028
Application：

WB, FCM, ICC
Species Reactivity：

Recombinant protein

Information

Data

Information

Product Name	GFP Tag Mouse mAb
Aliases	GFP; yfp; Green fluorescent protein
Background	The GFP (Green Fluorescent Protein) tag is a 27 kDa fluorescent protein derived from the jellyfish Aequorea victoria. It emits bright green light (λ~509 nm) upon exposure to blue or UV light, enabling real-time visualization of tagged proteins in live or fixed cells without additional substrates. Widely used as a reporter gene, GFP-tagging allows non-invasive tracking of protein localization, expression dynamics, and interactions across diverse organisms (e.g., bacteria, mammalian cells). Its engineered variants (e.g., EGFP, mCherry) offer enhanced brightness, photostability, and spectral versatility for multiplex imaging.
Application Information	Molecular Weight: Predicted, 27 kDa Clonality: Mouse monoclonal antibody Clone ID: 25GB7100 Species Reactivity: Recombinant protein Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen	Recombinant protein of GFP
Isotype	Mouse IgG1
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:4,000-1:20,000 Flow Cytometry (FCM): 1:200-1:2,000 Immunocytochemistry (ICC): 1:100-1:1,000
Note	This product is for research use only.

Data

Western blotting analysis using GFP-tag antibody (Cat#T028). Recombinant GFP (100 ng) expressed by E. coli were loaded and separated by SDS-PAGE. The blot was incubated with GFP-tag antibody (Cat#T028, 1:20,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of 293T cells transfected with GFP protein using GFP antibody (Cat#T028, 1:1,000). Nuclei were stained blue with DAPI; GFP was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
Flow cytometric analysis of 293T cells transfected with GFP protein using GFP antibody (Cat#T028, 1:2,000). Green, isotype control; red, GFP.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$69.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：
Catalog：

T028
Application：

WB, FCM, ICC
Species Reactivity：

Recombinant protein

Datasheet

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