Western blotting analysis using Phospho-RPA2 (T21) antibody (Cat#1123). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-RPA2 (T21) antibody (Cat#1123, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Phospho-RPA2 (T21) expression in HepG2 cells using Phospho-RPA2 (T21) antibody (Cat#1123, 1:2,000). Green, isotype control; red, Phospho-RPA2 (T21).
Immunocytochemical staining of HepG2 cells with Phospho-RPA2 (T21) antibody (Cat#1123, 1:1,000). Nuclei were stained blue with DAPI; Phospho-RPA2 (T21) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
RPA2; Replication Protein A2; Replication Protein A 32 KDa Subunit; Replication Protein A 34 KDa Subunit; Replication Factor A Protein 2; RF-A Protein 2; RP-A P32; RP-A P34; REPA2; RPA32; Replication Protein A2 (32kD); Replication Protein A2, 32kDa; RPA34
Western blotting analysis using Phospho-RPA2 (T21) antibody (Cat#1123). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-RPA2 (T21) antibody (Cat#1123, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Phospho-RPA2 (T21) expression in HepG2 cells using Phospho-RPA2 (T21) antibody (Cat#1123, 1:2,000). Green, isotype control; red, Phospho-RPA2 (T21).
Immunocytochemical staining of HepG2 cells with Phospho-RPA2 (T21) antibody (Cat#1123, 1:1,000). Nuclei were stained blue with DAPI; Phospho-RPA2 (T21) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using Phospho-RPA2 (T21) antibody (Cat#1123). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-RPA2 (T21) antibody (Cat#1123, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Phospho-RPA2 (T21) expression in HepG2 cells using Phospho-RPA2 (T21) antibody (Cat#1123, 1:2,000). Green, isotype control; red, Phospho-RPA2 (T21).
Immunocytochemical staining of HepG2 cells with Phospho-RPA2 (T21) antibody (Cat#1123, 1:1,000). Nuclei were stained blue with DAPI; Phospho-RPA2 (T21) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000647s ]
SELECT * FROM `goods` WHERE `code` = '1123' LIMIT 1 [ RunTime:0.000464s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000414s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000331s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000365s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 3657 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000355s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000526s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 3657 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000322s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000387s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000231s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.111448s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (5677,7130,5283,6941,4930,9855,7532,8608,7990,10334) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000961s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.003596s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000251s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.002089s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000253s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.002456s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000439s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000257s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000196s ]
