Western blotting analysis using GAPDH antibody (Cat#1226). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GAPDH antibody (Cat#1226, 1:50,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with GAPDH antibody(Cat#1226, 1:1,000). Nuclei were stained blue with DAPI; GAPDH was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Immunocytochemical staining of HepG2 cells with GAPDH antibody (Cat#1226, 1:1,000). Nuclei were stained blue with DAPI; GAPDH was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Scale bar, 20 μm.
Flow cytometric analysis of GAPDH expression in HepG2 cells using GAPDH antibody (Cat#1226, 1:2,000). Green, isotype control; red, GAPDH.