Western blotting analysis using WASL antibody (Cat#1312). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with WASL antibody (Cat#1312, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of WASL expression in HAP-1 cells using WASL antibody (Cat#1312, 1:2,000). Green, isotype control; red, WASL.
Immunocytochemical staining of HAP-1 cells with WASL antibody (Cat#1312, 1:1,000). Nuclei were stained blue with DAPI; WASL was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using WASL antibody (Cat#1312). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with WASL antibody (Cat#1312, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of WASL expression in HAP-1 cells using WASL antibody (Cat#1312, 1:2,000). Green, isotype control; red, WASL.
Immunocytochemical staining of HAP-1 cells with WASL antibody (Cat#1312, 1:1,000). Nuclei were stained blue with DAPI; WASL was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using WASL antibody (Cat#1312). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with WASL antibody (Cat#1312, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of WASL expression in HAP-1 cells using WASL antibody (Cat#1312, 1:2,000). Green, isotype control; red, WASL.
Immunocytochemical staining of HAP-1 cells with WASL antibody (Cat#1312, 1:1,000). Nuclei were stained blue with DAPI; WASL was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000673s ]
SELECT * FROM `goods` WHERE `code` = '1312' LIMIT 1 [ RunTime:0.000438s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000470s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000323s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000369s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 3732 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000323s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000443s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 3732 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000265s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000412s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000268s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.170440s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (8264,7951,7849,4625,6509,4875,8702,4137,8274,3696) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001144s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000492s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000198s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000460s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000237s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000300s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000390s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000210s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000189s ]
