Western blotting analysis using caspase 6 antibody (Cat#1372). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with caspase 6 antibody (Cat#1372, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using caspase 6 antibody (Cat#1372, 1:200). Antigen retrieval was done in EDTA buffer (pH 9.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HAP-1 cells with caspase 6 antibody (Cat#1372, 1:1,000). Nuclei were stained blue with DAPI; Caspase 6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Flow cytometric analysis of caspase 6 expression in HAP-1 cells using Caspase 6 antibody (Cat#1372, 1:2,000). Green, isotype control; red, Caspase 6.
Western blotting analysis using caspase 6 antibody (Cat#1372). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with caspase 6 antibody (Cat#1372, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using caspase 6 antibody (Cat#1372, 1:200). Antigen retrieval was done in EDTA buffer (pH 9.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HAP-1 cells with caspase 6 antibody (Cat#1372, 1:1,000). Nuclei were stained blue with DAPI; Caspase 6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Flow cytometric analysis of caspase 6 expression in HAP-1 cells using Caspase 6 antibody (Cat#1372, 1:2,000). Green, isotype control; red, Caspase 6.
Western blotting analysis using caspase 6 antibody (Cat#1372). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with caspase 6 antibody (Cat#1372, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using caspase 6 antibody (Cat#1372, 1:200). Antigen retrieval was done in EDTA buffer (pH 9.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HAP-1 cells with caspase 6 antibody (Cat#1372, 1:1,000). Nuclei were stained blue with DAPI; Caspase 6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Flow cytometric analysis of caspase 6 expression in HAP-1 cells using Caspase 6 antibody (Cat#1372, 1:2,000). Green, isotype control; red, Caspase 6.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.001784s ]
SELECT * FROM `goods` WHERE `code` = '1372' LIMIT 1 [ RunTime:0.001608s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.001011s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000877s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.001024s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 3753 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000770s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.002386s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 3753 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000601s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000512s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000237s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.062572s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (4301,7762,4431,6927,10171,6655,5593,6062,9344,4484) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000892s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000462s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000222s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000481s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000227s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000312s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000412s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000221s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000210s ]
