Western blotting analysis using FUBP1 antibody (Cat#1992). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FUBP1 antibody (Cat#1992, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human prostatic adenocarcinoma using FUBP1 antibody (Cat#1992, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Immunocytochemical staining of HepG2 cells with FUBP1 antibody (Cat#1992, 1:1,000). Nuclei were stained blue with DAPI; FUBP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Flow cytometric analysis of FUBP1 expression in HepG2 cells using FUBP1 antibody (Cat#1992, 1:2,000). Green, isotype control; red, FUBP1.
FUBP1; Far Upstream Element Binding Protein 1; FBP; Far Upstream Element (FUSE) Binding Protein 1; Far Upstream Element-Binding Protein 1; FUSE Binding Protein 1; DNA Helicase V; HDH V; FUBP; Epididymis Secretory Sperm Binding Protein; FUSE-Binding Protein 1
Western blotting analysis using FUBP1 antibody (Cat#1992). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FUBP1 antibody (Cat#1992, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human prostatic adenocarcinoma using FUBP1 antibody (Cat#1992, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Immunocytochemical staining of HepG2 cells with FUBP1 antibody (Cat#1992, 1:1,000). Nuclei were stained blue with DAPI; FUBP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Flow cytometric analysis of FUBP1 expression in HepG2 cells using FUBP1 antibody (Cat#1992, 1:2,000). Green, isotype control; red, FUBP1.
Western blotting analysis using FUBP1 antibody (Cat#1992). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FUBP1 antibody (Cat#1992, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human prostatic adenocarcinoma using FUBP1 antibody (Cat#1992, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Immunocytochemical staining of HepG2 cells with FUBP1 antibody (Cat#1992, 1:1,000). Nuclei were stained blue with DAPI; FUBP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Flow cytometric analysis of FUBP1 expression in HepG2 cells using FUBP1 antibody (Cat#1992, 1:2,000). Green, isotype control; red, FUBP1.
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