Western blotting analysis using HSPA14 antibody (Cat#2218). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HSPA14 antibody (Cat#2218, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human melanoma using HSPA14 antibody (Cat#2218, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Immunocytochemical staining of HAP-1 cells with HSPA14 antibody (Cat#2218, 1:1,000). Nuclei were stained blue with DAPI; HSPA14 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of HSPA14 expression in HAP-1 cells using HSPA14 antibody (Cat#2218, 1:2,000). Green, isotype control; red, HSPA14.
HSPA14; Heat Shock Protein Family A (Hsp70) Member 14; HSP70L1; HSP70-4; Heat Shock 70 KDa Protein 14; Heat Shock Protein HSP60; HSP70-Like Protein 1; Heat Shock Protein Hsp70-Related Protein; Heat Shock 70kDa Protein 14; MSANTD7; HSP60
Western blotting analysis using HSPA14 antibody (Cat#2218). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HSPA14 antibody (Cat#2218, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human melanoma using HSPA14 antibody (Cat#2218, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Immunocytochemical staining of HAP-1 cells with HSPA14 antibody (Cat#2218, 1:1,000). Nuclei were stained blue with DAPI; HSPA14 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of HSPA14 expression in HAP-1 cells using HSPA14 antibody (Cat#2218, 1:2,000). Green, isotype control; red, HSPA14.
Western blotting analysis using HSPA14 antibody (Cat#2218). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HSPA14 antibody (Cat#2218, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human melanoma using HSPA14 antibody (Cat#2218, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Immunocytochemical staining of HAP-1 cells with HSPA14 antibody (Cat#2218, 1:1,000). Nuclei were stained blue with DAPI; HSPA14 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of HSPA14 expression in HAP-1 cells using HSPA14 antibody (Cat#2218, 1:2,000). Green, isotype control; red, HSPA14.
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