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  • Primary Antibody
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Home>Product>Primary Antibody

P4HTM Recombinant Rabbit mAb

#2278

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Recombinant Antibody
Western blotting analysis using P4HTM antibody (Cat#2278). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with P4HTM antibody (Cat#2278, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of P4HTM expression in HepG2 cells using P4HTM antibody (Cat#2278, 1:2,000). Green, isotype control; red, P4HTM.
Immunocytochemical staining of HepG2 cells with P4HTM antibody (Cat#2278, 1:1,000). Nuclei were stained blue with DAPI; P4HTM was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Please select the specifications of the product.
Specification：
20 μL 100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    P4HTM
  • Catalog：
    2278
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name P4HTM Recombinant Rabbit mAb
Aliases Prolyl 4-Hydroxylase, Transmembrane; P4H-TM; PH4; HIFPH4; EGLN4; PHD4; PH-4; Prolyl 4-Hydroxylase, Transmembrane (Endoplasmic Reticulum); Hypoxia-Inducible Factor Prolyl Hydroxylase 4; Prolyl Hydroxlase Domain-Containing 4; Transmembrane Prolyl 4-Hydroxylase; HIF-Prolyl Hydroxylase 4; FLJ20262; HIF-PH4; HPH-4; Hypoxia Inducible Factor Prolyl 4 Hydroxylase; Hypoxia-Inducible Factor Prolyl 4-Hydroxylase; P4H With Transmembrane Domain; Proline 4-Hydroxylase; EC 1.14.11.29; HIDEA
Background

Gene Name: P4HTM

NCBI Gene Entry: 54681

UniProt Entry: Q9NXG6

Application Information

Molecular Weight: Predicted, 57 kDa, observed, 47 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB100

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human HIF Prolyl Hydroxylases
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for 1 year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using P4HTM antibody (Cat#2278). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with P4HTM antibody (Cat#2278, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of P4HTM expression in HepG2 cells using P4HTM antibody (Cat#2278, 1:2,000). Green, isotype control; red, P4HTM.
  • Immunocytochemical staining of HepG2 cells with P4HTM antibody (Cat#2278, 1:1,000). Nuclei were stained blue with DAPI; P4HTM was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Please select the specifications of the product.
Specification：
20 μL,100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    P4HTM
  • Catalog：
    2278
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using P4HTM antibody (Cat#2278). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with P4HTM antibody (Cat#2278, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of P4HTM expression in HepG2 cells using P4HTM antibody (Cat#2278, 1:2,000). Green, isotype control; red, P4HTM.
Immunocytochemical staining of HepG2 cells with P4HTM antibody (Cat#2278, 1:1,000). Nuclei were stained blue with DAPI; P4HTM was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Shopping cart (12)
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Estimated total order price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$23400
View the shopping cart Empty the shopping cart
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  149. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/facade/Route.php ( 4.70 KB )
  150. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-multi-app/src/Url.php ( 7.52 KB )
  151. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/route/Url.php ( 14.07 KB )
  152. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/head.php ( 3.43 KB )
  153. /www/wwwroot/sbywcs.dsger.com/app/web/view/product/detail.php ( 31.31 KB )
  154. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/public_cart.php ( 3.88 KB )
  155. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/foot.php ( 5.05 KB )
  156. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/Response.php ( 8.81 KB )
  157. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/response/Html.php ( 0.97 KB )
  158. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-trace/src/Html.php ( 4.42 KB )
  1. CONNECT:[ UseTime:0.000407s ] mysql:host=127.0.0.1;port=3306;dbname=sbywcs_dsger_com;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000966s ]
  3. SELECT * FROM `goods` WHERE `code` = '2278' LIMIT 1 [ RunTime:0.000537s ]
  4. SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000444s ]
  5. SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000309s ]
  6. SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000361s ]
  7. SELECT * FROM `goods_album` WHERE `goods_id` = 4243 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000379s ]
  8. SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000417s ]
  9. SELECT * FROM `goods_quotation` WHERE `goods_id` = 4243 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000249s ]
  10. SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000370s ]
  11. SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000219s ]
  12. SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.074852s ]
  13. SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (8019,8872,7354,5396,7195,4886,10161,8511,9381,5630) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000910s ]
  14. SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000453s ]
  15. SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000196s ]
  16. SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000458s ]
  17. SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000202s ]
  18. SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000291s ]
  19. SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000427s ]
  20. SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000224s ]
  21. SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000189s ]
0.113673s