Western blotting analysis using MBD3 antibody (Cat#2406). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MBD3 antibody (Cat#2406, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of MBD3 expression in HAP-1 cells using MBD3 antibody (Cat#2406, 1:2,000). Green, isotype control; red, MBD3.
Immunocytochemical staining of HAP-1 cells with MBD3 antibody (Cat#2406, 1:1,000). Nuclei were stained blue with DAPI; MBD3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using MBD3 antibody (Cat#2406). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MBD3 antibody (Cat#2406, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of MBD3 expression in HAP-1 cells using MBD3 antibody (Cat#2406, 1:2,000). Green, isotype control; red, MBD3.
Immunocytochemical staining of HAP-1 cells with MBD3 antibody (Cat#2406, 1:1,000). Nuclei were stained blue with DAPI; MBD3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using MBD3 antibody (Cat#2406). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MBD3 antibody (Cat#2406, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of MBD3 expression in HAP-1 cells using MBD3 antibody (Cat#2406, 1:2,000). Green, isotype control; red, MBD3.
Immunocytochemical staining of HAP-1 cells with MBD3 antibody (Cat#2406, 1:1,000). Nuclei were stained blue with DAPI; MBD3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.002188s ]
SELECT * FROM `goods` WHERE `code` = '2406' LIMIT 1 [ RunTime:0.005717s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000510s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000347s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000397s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 4333 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001036s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.004455s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 4333 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000310s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000850s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000247s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.171480s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (10157,8257,4174,4170,10381,216,8657,7923,5457,6522) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.002021s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.010217s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.001942s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.002177s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000250s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.006179s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.002270s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.004442s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.002098s ]
