Western blotting analysis using Raptor antibody (Cat#2441). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Raptor antibody (Cat#2441, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Raptor expression in C2C12 cells using Raptor antibody (Cat#2441, 1:2,000). Green, isotype control; red, Raptor.
Regulatory Associated Protein Of MTOR Complex 1; Raptor; KIAA1303; KOG1; Mip1; Regulatory-Associated Protein Of MTOR; P150 Target Of Rapamycin (TOR)-Scaffold Protein Containing WD-Repeats; Regulatory Associated Protein Of MTOR, Complex 1; P150 Target Of Rapamycin (TOR)-Scaffold Protein; Regulatory Associated Protein Of MTOR; RAPTOR
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
A synthesized peptide derived from human Raptor
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using Raptor antibody (Cat#2441). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Raptor antibody (Cat#2441, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Raptor expression in C2C12 cells using Raptor antibody (Cat#2441, 1:2,000). Green, isotype control; red, Raptor.
Western blotting analysis using Raptor antibody (Cat#2441). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Raptor antibody (Cat#2441, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Raptor expression in C2C12 cells using Raptor antibody (Cat#2441, 1:2,000). Green, isotype control; red, Raptor.
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