Western blotting analysis using phospho-LAT (Y220) antibody (Cat#2553). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-LAT (Y220) antibody (Cat#2553, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with Phospho-LAT (Y191) antibody (Cat#2553, 1:1,000). Nuclei were stained blue with DAPI; Phospho-LAT (Y191) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：Medium. Scale bar, 20 μm.
LAT; Linker For Activation Of T Cells; LAT1; Linker For Activation Of T Cells, Transmembrane Adaptor; Linker For Activation Of T-Cells Family Member 1; 36 KDa Phosphotyrosine Adapter Protein; P36-38; Pp36; 36 KDa Phospho-Tyrosine Adapter Protein; 36 KDa Phospho-Tyrosine Adaptor Protein; IMD52
Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen
A synthesized peptide derived from human Phospho-LAT (Y220)
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (ICC): 1:100-1:1,000
Note
This product is for research use only.
Data
Western blotting analysis using phospho-LAT (Y220) antibody (Cat#2553). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-LAT (Y220) antibody (Cat#2553, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with Phospho-LAT (Y191) antibody (Cat#2553, 1:1,000). Nuclei were stained blue with DAPI; Phospho-LAT (Y191) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：Medium. Scale bar, 20 μm.
Western blotting analysis using phospho-LAT (Y220) antibody (Cat#2553). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-LAT (Y220) antibody (Cat#2553, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with Phospho-LAT (Y191) antibody (Cat#2553, 1:1,000). Nuclei were stained blue with DAPI; Phospho-LAT (Y191) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：Medium. Scale bar, 20 μm.
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