Western blotting analysis using STAT5A/B antibody (Cat#2647). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with STAT5A/B antibody (Cat#2647, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of STAT5A/B expression in HepG2 cells using STAT5A/B antibody (Cat#2647, 1:2,000). Green, isotype control; red, STAT5A/B.
Immunocytochemical staining of HepG2 cells with STAT5A/B antibody (Cat#2647, 1:1,000). Nuclei were stained blue with DAPI;STAT5A/B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using STAT5A/B antibody (Cat#2647, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
STAT5A; Signal Transducer And Activator Of Transcription 5A; STAT5; MGF; Epididymis Secretory Sperm Binding Protein; TAT5B; Signal Transducer And Activator Of Transcription 5B; Transcription Factor STAT5B; GHISID2; STAT5
Western blotting analysis using STAT5A/B antibody (Cat#2647). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with STAT5A/B antibody (Cat#2647, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of STAT5A/B expression in HepG2 cells using STAT5A/B antibody (Cat#2647, 1:2,000). Green, isotype control; red, STAT5A/B.
Immunocytochemical staining of HepG2 cells with STAT5A/B antibody (Cat#2647, 1:1,000). Nuclei were stained blue with DAPI;STAT5A/B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using STAT5A/B antibody (Cat#2647, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Western blotting analysis using STAT5A/B antibody (Cat#2647). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with STAT5A/B antibody (Cat#2647, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of STAT5A/B expression in HepG2 cells using STAT5A/B antibody (Cat#2647, 1:2,000). Green, isotype control; red, STAT5A/B.
Immunocytochemical staining of HepG2 cells with STAT5A/B antibody (Cat#2647, 1:1,000). Nuclei were stained blue with DAPI;STAT5A/B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using STAT5A/B antibody (Cat#2647, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
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