Western blotting analysis using DNA topoisomerase I antibody (Cat#2704). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DNA topoisomerase I antibody (Cat#2704, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of DNA topoisomerase I expression in HT-1080 cells using DNA topoisomerase I antibody (Cat#2704, 1:2,000). Green, isotype control; red, DNA topoisomerase I.
Immunocytochemical staining of HT-1080 cells with DNA topoisomerase I antibody (Cat#2704, 1:1,000). Nuclei were stained blue with DAPI; DNA topoisomerase I was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using DNA topoisomerase I antibody (Cat#2704). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DNA topoisomerase I antibody (Cat#2704, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of DNA topoisomerase I expression in HT-1080 cells using DNA topoisomerase I antibody (Cat#2704, 1:2,000). Green, isotype control; red, DNA topoisomerase I.
Immunocytochemical staining of HT-1080 cells with DNA topoisomerase I antibody (Cat#2704, 1:1,000). Nuclei were stained blue with DAPI; DNA topoisomerase I was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using DNA topoisomerase I antibody (Cat#2704). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DNA topoisomerase I antibody (Cat#2704, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of DNA topoisomerase I expression in HT-1080 cells using DNA topoisomerase I antibody (Cat#2704, 1:2,000). Green, isotype control; red, DNA topoisomerase I.
Immunocytochemical staining of HT-1080 cells with DNA topoisomerase I antibody (Cat#2704, 1:1,000). Nuclei were stained blue with DAPI; DNA topoisomerase I was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000781s ]
SELECT * FROM `goods` WHERE `code` = '2704' LIMIT 1 [ RunTime:0.000521s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000431s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000342s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000382s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 4509 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000372s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000456s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 4509 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000262s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000437s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000250s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.076005s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (3849,5056,3922,4247,6115,3972,8213,9463,4852,8077) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001165s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000650s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000337s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.001153s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.001911s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.002510s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000622s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000466s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000298s ]
