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  • Primary Antibody
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Home>Product>Primary Antibody

ACTA2 Recombinant Rabbit mAb

#2852

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Western blotting analysis using alpha smooth muscle Actin antibody (Cat#2852). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with alpha smooth muscle Actin antibody (Cat#2852, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of α smooth muscle Actin expression in C2C12 cells using α smooth muscle Actin antibody (Cat#2852, 1:2,000). Green, isotype control; red, α smooth muscle Actin.
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using alpha smooth muscle actin antibody (Cat#2852, 1:500). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Please select the specifications of the product.
Specification：
20 μL 100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    ACTA2
  • Catalog：
    2852
  • Application：
    WB, FCM, IHC-P
  • Species Reactivity：
    M, R

Information

Data

Information
Product Name ACTA2 Recombinant Rabbit mAb
Aliases ACTA2; Actin Alpha 2, Smooth Muscle; ACTSA; Cell Growth-Inhibiting Gene 46 Protein; Actin, Alpha 2, Smooth Muscle, Aorta; Actin, Aortic Smooth Muscle; Alpha-Cardiac Actin; Alpha-Actin-2; EC 3.6.4.-; ACTVS; α-SMA; alpha smooth muscle Actin; a SMA
Background

Gene Name: ACTA2

NCBI Gene Entry: 59

UniProt Entry: P62736

Application Information

Molecular Weight: Predicted, 42 kDa, observed, 43 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB2845

Species Reactivity: Mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunohistochemistry-Paraffin (IHC-P)
Immunogen Recombinant protein of human Smooth Muscle Actin
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for 1 year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunohistochemistry-Paraffin (IHC-P): 1:250-1:500
Note This product is for research use only.
Data
  • Western blotting analysis using alpha smooth muscle Actin antibody (Cat#2852). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with alpha smooth muscle Actin antibody (Cat#2852, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of α smooth muscle Actin expression in C2C12 cells using α smooth muscle Actin antibody (Cat#2852, 1:2,000). Green, isotype control; red, α smooth muscle Actin.
  • Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using alpha smooth muscle actin antibody (Cat#2852, 1:500). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Please select the specifications of the product.
Specification：
20 μL,100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    ACTA2
  • Catalog：
    2852
  • Application：
    WB, FCM, IHC-P
  • Species Reactivity：
    M, R
RELATED PRODUCTS
Western blotting analysis using alpha smooth muscle Actin antibody (Cat#2852). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with alpha smooth muscle Actin antibody (Cat#2852, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of α smooth muscle Actin expression in C2C12 cells using α smooth muscle Actin antibody (Cat#2852, 1:2,000). Green, isotype control; red, α smooth muscle Actin.
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using alpha smooth muscle actin antibody (Cat#2852, 1:500). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Shopping cart (12)
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Estimated total order price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$23400
View the shopping cart Empty the shopping cart
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  • Email Address

    support@genuinbiotech.com

    sales@genuinbiotech.com

  • Office Hours

    8:30 AM - 5:30 PM EST Mon-Fri

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  150. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-multi-app/src/Url.php ( 7.52 KB )
  151. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/route/Url.php ( 14.07 KB )
  152. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/head.php ( 3.43 KB )
  153. /www/wwwroot/sbywcs.dsger.com/app/web/view/product/detail.php ( 31.31 KB )
  154. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/public_cart.php ( 3.88 KB )
  155. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/foot.php ( 5.05 KB )
  156. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/Response.php ( 8.81 KB )
  157. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/response/Html.php ( 0.97 KB )
  158. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-trace/src/Html.php ( 4.42 KB )
  1. CONNECT:[ UseTime:0.000443s ] mysql:host=127.0.0.1;port=3306;dbname=sbywcs_dsger_com;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000830s ]
  3. SELECT * FROM `goods` WHERE `code` = '2852' LIMIT 1 [ RunTime:0.000512s ]
  4. SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000453s ]
  5. SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000347s ]
  6. SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000405s ]
  7. SELECT * FROM `goods_album` WHERE `goods_id` = 4603 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000403s ]
  8. SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000443s ]
  9. SELECT * FROM `goods_quotation` WHERE `goods_id` = 4603 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000340s ]
  10. SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000413s ]
  11. SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000317s ]
  12. SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.055882s ]
  13. SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (7981,3738,7930,9549,7460,233,7936,7733,6166,4994) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000873s ]
  14. SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000480s ]
  15. SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000206s ]
  16. SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000488s ]
  17. SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000232s ]
  18. SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000303s ]
  19. SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000377s ]
  20. SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000294s ]
  21. SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000212s ]
0.099574s