Western blotting analysis using ICAM3 / CD50 antibody (Cat#2892). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ICAM3 / CD50 antibody (Cat#2892, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
A synthesized peptide derived from human ICAM3 / CD50
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000
Note
This product is for research use only.
Data
Western blotting analysis using ICAM3 / CD50 antibody (Cat#2892). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ICAM3 / CD50 antibody (Cat#2892, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using ICAM3 / CD50 antibody (Cat#2892). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ICAM3 / CD50 antibody (Cat#2892, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.002830s ]
SELECT * FROM `goods` WHERE `code` = '2892' LIMIT 1 [ RunTime:0.002572s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000490s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000337s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000387s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 4637 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000352s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000543s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 4637 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000258s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000402s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000243s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.090889s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (9687,8545,6873,5944,7463,7596,8710,7200,7747,6753) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001642s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000694s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000386s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000985s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000264s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000315s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000703s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000230s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000218s ]
