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  • Primary Antibody
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Home>Product>Primary Antibody

H2B Clustered Histone 12 Recombinant Rabbit mAb

#2949

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Recombinant Antibody
Western blotting analysis using histone H2B antibody (Cat#2949). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with histone H2B antibody (Cat#2949, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using histone H2B antibody (Cat#2949, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HepG2 cells with histone H2B antibody (Cat#2949, 1:1,000). Nuclei were stained blue with DAPI; Histone H2B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Please select the specifications of the product.
Specification：
20 μL 100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    H2BC12
  • Catalog：
    2949
  • Application：
    WB, ICC, IHC-P
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name H2B Clustered Histone 12 Recombinant Rabbit mAb
Aliases H2BC12; H2B Clustered Histone 12; HIST1H2B; H2BFAiii; H2BFT; Histone Cluster 1 H2B Family Member K; H2B Histone Family, Member T; HIRA-Interacting Protein 1; Histone Cluster 1, H2bk; Histone H2B Type 1-K; Histone 1, H2bk; H2B K; Histone Family Member; HIRIP1; H2B/S; H2BK
Background

Gene Name: H2BC12

NCBI Gene Entry: 85236

UniProt Entry: O60814

Application Information

Molecular Weight: Predicted, 14 kDa, observed, 17 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB3315

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), immunocytochemistry (ICC), immunohistochemistry-paraffin (IHC-P)
Immunogen A synthesized peptide derived from human Histone H2B
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for 1 year.
Recommended Dilutions Western Blotting (WB): 1:4,000-1:20,000
Immunocytochemistry (ICC): 1:100-1:1,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using histone H2B antibody (Cat#2949). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with histone H2B antibody (Cat#2949, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using histone H2B antibody (Cat#2949, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunocytochemical staining of HepG2 cells with histone H2B antibody (Cat#2949, 1:1,000). Nuclei were stained blue with DAPI; Histone H2B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Please select the specifications of the product.
Specification：
20 μL,100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    H2BC12
  • Catalog：
    2949
  • Application：
    WB, ICC, IHC-P
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using histone H2B antibody (Cat#2949). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with histone H2B antibody (Cat#2949, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using histone H2B antibody (Cat#2949, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HepG2 cells with histone H2B antibody (Cat#2949, 1:1,000). Nuclei were stained blue with DAPI; Histone H2B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Shopping cart (12)
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Estimated total order price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$23400
View the shopping cart Empty the shopping cart
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  • Email Address

    support@genuinbiotech.com

    sales@genuinbiotech.com

  • Office Hours

    8:30 AM - 5:30 PM EST Mon-Fri

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All Rights Reserved.

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  147. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/facade/Request.php ( 9.20 KB )
  148. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/mobile.php ( 4.08 KB )
  149. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/facade/Route.php ( 4.70 KB )
  150. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-multi-app/src/Url.php ( 7.52 KB )
  151. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/route/Url.php ( 14.07 KB )
  152. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/head.php ( 3.43 KB )
  153. /www/wwwroot/sbywcs.dsger.com/app/web/view/product/detail.php ( 31.31 KB )
  154. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/public_cart.php ( 3.88 KB )
  155. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/foot.php ( 5.05 KB )
  156. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/Response.php ( 8.81 KB )
  157. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/response/Html.php ( 0.97 KB )
  158. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-trace/src/Html.php ( 4.42 KB )
  1. CONNECT:[ UseTime:0.000527s ] mysql:host=127.0.0.1;port=3306;dbname=sbywcs_dsger_com;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000854s ]
  3. SELECT * FROM `goods` WHERE `code` = '2949' LIMIT 1 [ RunTime:0.000543s ]
  4. SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000558s ]
  5. SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000391s ]
  6. SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000436s ]
  7. SELECT * FROM `goods_album` WHERE `goods_id` = 4692 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000461s ]
  8. SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000501s ]
  9. SELECT * FROM `goods_quotation` WHERE `goods_id` = 4692 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000315s ]
  10. SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000387s ]
  11. SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000254s ]
  12. SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.052704s ]
  13. SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (8763,10465,9094,5404,7568,7163,7137,8743,6730,7023) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000830s ]
  14. SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000479s ]
  15. SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000227s ]
  16. SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000425s ]
  17. SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000264s ]
  18. SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000300s ]
  19. SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000392s ]
  20. SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000236s ]
  21. SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000216s ]
0.093547s