Western blotting analysis using Hsp27 antibody (Cat#2953). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Hsp27 antibody (Cat#2953, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Hsp27 expression in HepG2 cells using Hsp27 antibody (Cat#2953, 1:2,000). Green, isotype control; red, Hsp27.
Immunocytochemical staining of HepG2 cells with Hsp27 antibody (Cat#2953, 1:1,000). Nuclei were stained blue with DAPI; Hsp27 was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using Hsp27 antibody (Cat#2953). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Hsp27 antibody (Cat#2953, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Hsp27 expression in HepG2 cells using Hsp27 antibody (Cat#2953, 1:2,000). Green, isotype control; red, Hsp27.
Immunocytochemical staining of HepG2 cells with Hsp27 antibody (Cat#2953, 1:1,000). Nuclei were stained blue with DAPI; Hsp27 was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using Hsp27 antibody (Cat#2953). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Hsp27 antibody (Cat#2953, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Hsp27 expression in HepG2 cells using Hsp27 antibody (Cat#2953, 1:2,000). Green, isotype control; red, Hsp27.
Immunocytochemical staining of HepG2 cells with Hsp27 antibody (Cat#2953, 1:1,000). Nuclei were stained blue with DAPI; Hsp27 was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: Medium. Scale bar, 20 μm.
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