Western blotting analysis using phospho-p53 (S9) antibody (Cat#3072). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-p53 (S9) antibody (Cat#3072, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of phospho-p53 (S9) expression in HeLa cells using phospho-p53 (S9) antibody (Cat#3072, 1:2,000). Green, isotype control; red, phospho-p53 (S9).
Immunocytochemical staining of Hela cells with phospho-p53 (S9) antibody (Cat#3072, 1:1,000). Nuclei were stained blue with DAPI; Phospho-p53 (S9) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using phospho-p53 (S9) antibody (Cat#3072). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-p53 (S9) antibody (Cat#3072, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of phospho-p53 (S9) expression in HeLa cells using phospho-p53 (S9) antibody (Cat#3072, 1:2,000). Green, isotype control; red, phospho-p53 (S9).
Immunocytochemical staining of Hela cells with phospho-p53 (S9) antibody (Cat#3072, 1:1,000). Nuclei were stained blue with DAPI; Phospho-p53 (S9) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using phospho-p53 (S9) antibody (Cat#3072). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-p53 (S9) antibody (Cat#3072, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of phospho-p53 (S9) expression in HeLa cells using phospho-p53 (S9) antibody (Cat#3072, 1:2,000). Green, isotype control; red, phospho-p53 (S9).
Immunocytochemical staining of Hela cells with phospho-p53 (S9) antibody (Cat#3072, 1:1,000). Nuclei were stained blue with DAPI; Phospho-p53 (S9) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
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