Western blotting analysis using phospho-PAK1/2/3 (S144+S141+S154) antibody (Cat#3079). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-PAK1/2/3 (S144+S141+S154) antibody (Cat#3079, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Phospho-PAK1/2/3 (S144+S141+S154) expression in H9c2 cells using Phospho-PAK1/2/3 (S144+S141+S154) antibody (Cat#3079, 1:2,000). Green, isotype control; red, Phospho-PAK1/2/3 (S144+S141+S154).
Immunocytochemical staining of H9C2 cells with Phospho-PAK1/2/3 (S144+S141+S139) antibody(Cat#3079, 1:1,000). Nuclei were stained blue with DAPI; Phospho-PAK1/2/3 (S144+S141+S139) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Scale bar, 20 μm.
Western blotting analysis using phospho-PAK1/2/3 (S144+S141+S154) antibody (Cat#3079). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-PAK1/2/3 (S144+S141+S154) antibody (Cat#3079, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Phospho-PAK1/2/3 (S144+S141+S154) expression in H9c2 cells using Phospho-PAK1/2/3 (S144+S141+S154) antibody (Cat#3079, 1:2,000). Green, isotype control; red, Phospho-PAK1/2/3 (S144+S141+S154).
Immunocytochemical staining of H9C2 cells with Phospho-PAK1/2/3 (S144+S141+S139) antibody(Cat#3079, 1:1,000). Nuclei were stained blue with DAPI; Phospho-PAK1/2/3 (S144+S141+S139) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Scale bar, 20 μm.
Western blotting analysis using phospho-PAK1/2/3 (S144+S141+S154) antibody (Cat#3079). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-PAK1/2/3 (S144+S141+S154) antibody (Cat#3079, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Phospho-PAK1/2/3 (S144+S141+S154) expression in H9c2 cells using Phospho-PAK1/2/3 (S144+S141+S154) antibody (Cat#3079, 1:2,000). Green, isotype control; red, Phospho-PAK1/2/3 (S144+S141+S154).
Immunocytochemical staining of H9C2 cells with Phospho-PAK1/2/3 (S144+S141+S139) antibody(Cat#3079, 1:1,000). Nuclei were stained blue with DAPI; Phospho-PAK1/2/3 (S144+S141+S139) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Scale bar, 20 μm.
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