Western blotting analysis using phospho-EGFR (Tyr1068) antibody (Cat#3177). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-EGFR (Tyr1068) antibody (Cat#3177, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of phospho-EGFR (Tyr1068) expression in HepG2 cells using phospho-EGFR (Tyr1068) antibody (Cat#3177, 1:2,000). Green, isotype control; red, phospho-EGFR (Tyr1068).
Western blotting analysis using anti-phospho-EGFR (Tyr1068) antibody (Cat#3177) antibody. Total cell lysates (20 ug) from various breast cancer cell lines were loaded and separated by SDS-PAGE. The membrane was incubated with the respective primary antibody and corresponding HRP-conjugated secondary antibody. Images were developed using Clarity Western ECL Substrate. For reference, we use the Bio-Rad Dual Protein Ladder and the provided ladder images are merged colorimetric. Included for each image is expected observed protein sizes, the antibody dilution, and chemiluminescent exposure time.