Western blotting analysis using HSP90AB1 antibody (Cat#3416). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HSP90AB1 antibody (Cat#3416, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
Flow cytometric analysis of HSP90AB1 expression in HepG2 cells using HSP90AB1 antibody (Cat#3416, 1:2,000). Green, isotype control; red, HSP90AB1.
HSP90AB1; Heat Shock Protein 90 Alpha Family Class B Member 1; HSPC2; HSPCB; Heat Shock Protein 90kDa Alpha (Cytosolic), Class B Member 1; Heat Shock 90kD Protein 1, Beta; Heat Shock Protein HSP 90-Beta; Heat Shock 84 KDa; HSP90B; HSP84; Heat Shock Protein 90kDa Alpha Family Class B Member 1; Heat Shock 90kDa Protein 1, Beta; Heat Shock Protein 90 KDa; HSP90-Beta; D6S182; HSP 90; HSP 84
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
Recombinant protein of human HSP90AB1
Isotype
Mouse IgG2b
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB):1:2,500-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using HSP90AB1 antibody (Cat#3416). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HSP90AB1 antibody (Cat#3416, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
Flow cytometric analysis of HSP90AB1 expression in HepG2 cells using HSP90AB1 antibody (Cat#3416, 1:2,000). Green, isotype control; red, HSP90AB1.
Western blotting analysis using HSP90AB1 antibody (Cat#3416). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HSP90AB1 antibody (Cat#3416, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
Flow cytometric analysis of HSP90AB1 expression in HepG2 cells using HSP90AB1 antibody (Cat#3416, 1:2,000). Green, isotype control; red, HSP90AB1.
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