Western blotting analysis using L1 cell adhesion molecule antibody (Cat#3421). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with L1 cell adhesion molecule antibody (Cat#3421, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
Flow cytometric analysis of L1 cell adhesion molecule expression in HepG2 cells using L1 cell adhesion molecule antibody (Cat#3421, 1:2,000). Green, isotype control; red, L1 cell adhesion molecule.
Immunocytochemical staining of HepG2 cells with L1 cell adhesion molecule antibody (Cat#3421, 1:1,000). Nuclei were stained blue with DAPI; L1 cell adhesion molecule was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：Medium. Scale bar, 20 μm.
Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen
Recombinant protein of human L1CAM
Isotype
Mouse IgG1
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB):1:500-1:2,500 Flow Cytometry (FCM): 1:200-1:2,000 Immunocytochemistry (ICC): 1:100-1:1,000
Note
This product is for research use only.
Data
Western blotting analysis using L1 cell adhesion molecule antibody (Cat#3421). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with L1 cell adhesion molecule antibody (Cat#3421, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
Flow cytometric analysis of L1 cell adhesion molecule expression in HepG2 cells using L1 cell adhesion molecule antibody (Cat#3421, 1:2,000). Green, isotype control; red, L1 cell adhesion molecule.
Immunocytochemical staining of HepG2 cells with L1 cell adhesion molecule antibody (Cat#3421, 1:1,000). Nuclei were stained blue with DAPI; L1 cell adhesion molecule was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：Medium. Scale bar, 20 μm.
Western blotting analysis using L1 cell adhesion molecule antibody (Cat#3421). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with L1 cell adhesion molecule antibody (Cat#3421, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
Flow cytometric analysis of L1 cell adhesion molecule expression in HepG2 cells using L1 cell adhesion molecule antibody (Cat#3421, 1:2,000). Green, isotype control; red, L1 cell adhesion molecule.
Immunocytochemical staining of HepG2 cells with L1 cell adhesion molecule antibody (Cat#3421, 1:1,000). Nuclei were stained blue with DAPI; L1 cell adhesion molecule was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：Medium. Scale bar, 20 μm.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000805s ]
SELECT * FROM `goods` WHERE `code` = '3421' LIMIT 1 [ RunTime:0.000662s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.001174s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000918s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000561s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 5033 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000760s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000627s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 5033 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000247s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000827s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000222s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.126712s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (4906,9415,4759,4626,8423,4144,9934,9102,3931,3703) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.002330s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000822s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000212s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000469s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000217s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.002505s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000642s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000528s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000726s ]
