Western blotting analysis using O-6-methylguanine-DNA methyltransferase antibody (Cat#3467). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with O-6-methylguanine-DNA methyltransferase antibody (Cat#3467, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000
Note
This product is for research use only.
Data
Western blotting analysis using O-6-methylguanine-DNA methyltransferase antibody (Cat#3467). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with O-6-methylguanine-DNA methyltransferase antibody (Cat#3467, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using O-6-methylguanine-DNA methyltransferase antibody (Cat#3467). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with O-6-methylguanine-DNA methyltransferase antibody (Cat#3467, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.002083s ]
SELECT * FROM `goods` WHERE `code` = '3467' LIMIT 1 [ RunTime:0.002377s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.001498s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.002452s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.001356s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 5057 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.002071s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.002411s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 5057 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000772s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.001278s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000910s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.193492s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (3826,9020,6148,4968,8790,5983,6934,191,8963,8150) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001454s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.005158s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000613s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.003571s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.003043s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000668s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.003553s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.002268s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.001221s ]
