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  • Primary Antibody
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Home>Product>Primary Antibody

Interleukin 12B Mouse mAb

#3483

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Western blotting analysis using interleukin 12B antibody (Cat#3483). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with interleukin 12B antibody (Cat#3483, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
Flow cytometric analysis of Interleukin 12B expression in HAP-1 cells using Interleukin 12B antibody (Cat#3483, 1:2,000). Green, isotype control; red, Interleukin 12B.
Immunocytochemical staining of HAP-1 cells with Interleukin 12B antibody (Cat#3483, 1:1,000). Nuclei were stained blue with DAPI; Interleukin 12B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Please select the specifications of the product.
Specification：
20 μL 100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    IL12B
  • Catalog：
    3483
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, R

Information

Data

Information
Product Name Interleukin 12B Mouse mAb
Aliases IL12B; Interleukin 12B; IL-12B; CLMF2; NKSF2; CLMF; NKSF; Interleukin 12B (Natural Killer Cell Stimulatory Factor 2, Cytotoxic Lymphocyte Maturation Factor 2, P40); Natural Killer Cell Stimulatory Factor, 40 KD Subunit; Cytotoxic Lymphocyte Maturation Factor 40 KDa Subunit; NK Cell Stimulatory Factor Chain; Interleukin-12 Subunit Beta; Interleukin-12 Beta Chain; Interleukin 12, P40; IL12, Subunit P40; IL-12 Subunit P40; CLMF P40; Cytotoxic Lymphocyte Maturation Factor 2, P40; Natural Killer Cell Stimulatory Factor-2; IMD28; IMD29
Background

Gene Name: IL12B

NCBI Gene Entry: 3593

UniProt Entry: P29460

Application Information

Molecular Weight: Predicted, 37 kDa, observed, 40 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB5840

Species Reactivity: Human,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human IL12B
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for 1 year.
Recommended Dilutions Western Blotting (WB):1:2,500-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using interleukin 12B antibody (Cat#3483). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with interleukin 12B antibody (Cat#3483, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
  • Flow cytometric analysis of Interleukin 12B expression in HAP-1 cells using Interleukin 12B antibody (Cat#3483, 1:2,000). Green, isotype control; red, Interleukin 12B.
  • Immunocytochemical staining of HAP-1 cells with Interleukin 12B antibody (Cat#3483, 1:1,000). Nuclei were stained blue with DAPI; Interleukin 12B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Please select the specifications of the product.
Specification：
20 μL,100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    IL12B
  • Catalog：
    3483
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, R
RELATED PRODUCTS
Western blotting analysis using interleukin 12B antibody (Cat#3483). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with interleukin 12B antibody (Cat#3483, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
Flow cytometric analysis of Interleukin 12B expression in HAP-1 cells using Interleukin 12B antibody (Cat#3483, 1:2,000). Green, isotype control; red, Interleukin 12B.
Immunocytochemical staining of HAP-1 cells with Interleukin 12B antibody (Cat#3483, 1:1,000). Nuclei were stained blue with DAPI; Interleukin 12B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Shopping cart (12)
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Estimated total order price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$23400
View the shopping cart Empty the shopping cart
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  149. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/facade/Route.php ( 4.70 KB )
  150. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-multi-app/src/Url.php ( 7.52 KB )
  151. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/route/Url.php ( 14.07 KB )
  152. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/head.php ( 3.43 KB )
  153. /www/wwwroot/sbywcs.dsger.com/app/web/view/product/detail.php ( 31.31 KB )
  154. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/public_cart.php ( 3.88 KB )
  155. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/foot.php ( 5.05 KB )
  156. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/Response.php ( 8.81 KB )
  157. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/response/Html.php ( 0.97 KB )
  158. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-trace/src/Html.php ( 4.42 KB )
  1. CONNECT:[ UseTime:0.000461s ] mysql:host=127.0.0.1;port=3306;dbname=sbywcs_dsger_com;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000780s ]
  3. SELECT * FROM `goods` WHERE `code` = '3483' LIMIT 1 [ RunTime:0.001313s ]
  4. SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000541s ]
  5. SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000910s ]
  6. SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000899s ]
  7. SELECT * FROM `goods_album` WHERE `goods_id` = 5065 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000716s ]
  8. SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.002007s ]
  9. SELECT * FROM `goods_quotation` WHERE `goods_id` = 5065 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000427s ]
  10. SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000935s ]
  11. SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000635s ]
  12. SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.125213s ]
  13. SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (159,4499,9078,10158,3987,6782,5757,9711,4118,7942) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001287s ]
  14. SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000731s ]
  15. SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000316s ]
  16. SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000751s ]
  17. SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000388s ]
  18. SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000490s ]
  19. SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000615s ]
  20. SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000400s ]
  21. SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000333s ]
0.196371s