Western blotting analysis using BORA antibody (Cat#3680). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BORA antibody (Cat#3680, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of BORA expression in HepG2 cells using BORA antibody (Cat#3680, 1:2,000). Green, isotype control; red, BORA.
Immunocytochemical staining of HepG2 cells with BORA antibody (Cat#3680, 1:1,000). Nuclei were stained blue with DAPI; BORA was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
BORA; BORA Aurora Kinase A Activator; C13orf34; Protein Aurora Borealis; FLJ22624; Chromosome 13 Open Reading Frame 34; Bora, Aurora Kinase A Activator; HsBora
Western blotting analysis using BORA antibody (Cat#3680). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BORA antibody (Cat#3680, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of BORA expression in HepG2 cells using BORA antibody (Cat#3680, 1:2,000). Green, isotype control; red, BORA.
Immunocytochemical staining of HepG2 cells with BORA antibody (Cat#3680, 1:1,000). Nuclei were stained blue with DAPI; BORA was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using BORA antibody (Cat#3680). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BORA antibody (Cat#3680, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of BORA expression in HepG2 cells using BORA antibody (Cat#3680, 1:2,000). Green, isotype control; red, BORA.
Immunocytochemical staining of HepG2 cells with BORA antibody (Cat#3680, 1:1,000). Nuclei were stained blue with DAPI; BORA was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.001342s ]
SELECT * FROM `goods` WHERE `code` = '3680' LIMIT 1 [ RunTime:0.000533s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000426s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000353s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000385s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 5128 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000328s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.001404s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 5128 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000857s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000909s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000618s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.135589s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (10137,6822,4949,5780,7573,7313,164,7563,6382,4953) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.007466s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.004668s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.001305s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000778s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000750s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.002222s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.009577s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000335s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000208s ]