Western blotting analysis using SOX2 antibody (Cat#3711). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SOX2 antibody (Cat#3711, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of SOX2 expression in HAP-1 cells using SOX2 antibody (Cat#3711, 1:2,000). Green, isotype control; red, SOX2.
Immunocytochemical staining of HAP-1 cells with SOX2 antibody (Cat#3711, 1:1,000). Nuclei were stained blue with DAPI; SOX2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：High. Scale bar, 20 μm.
Western blotting analysis using SOX2 antibody (Cat#3711). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SOX2 antibody (Cat#3711, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of SOX2 expression in HAP-1 cells using SOX2 antibody (Cat#3711, 1:2,000). Green, isotype control; red, SOX2.
Immunocytochemical staining of HAP-1 cells with SOX2 antibody (Cat#3711, 1:1,000). Nuclei were stained blue with DAPI; SOX2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：High. Scale bar, 20 μm.
Western blotting analysis using SOX2 antibody (Cat#3711). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SOX2 antibody (Cat#3711, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of SOX2 expression in HAP-1 cells using SOX2 antibody (Cat#3711, 1:2,000). Green, isotype control; red, SOX2.
Immunocytochemical staining of HAP-1 cells with SOX2 antibody (Cat#3711, 1:1,000). Nuclei were stained blue with DAPI; SOX2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：High. Scale bar, 20 μm.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.001291s ]
SELECT * FROM `goods` WHERE `code` = '3711' LIMIT 1 [ RunTime:0.001604s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000910s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000548s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.001137s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 5146 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001292s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.001033s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 5146 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000326s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.001082s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000451s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.091758s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (4951,8094,10522,5509,7458,7233,4357,158,9425,6912) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000816s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000460s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000228s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000514s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000209s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000332s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000383s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000220s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000235s ]
