Western blotting analysis using DNA methyltransferase 3 alpha antibody (Cat#3760). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DNA methyltransferase 3 alpha antibody (Cat#3760, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of DNA methyltransferase 3 alpha expression in H9c2 cells using DNA methyltransferase 3 alpha antibody (Cat#3760, 1:2,000). Green, isotype control; red, DNA methyltransferase 3 alpha.
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
Recombinant protein of human DNMT3A
Isotype
Mouse IgG1 kappa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using DNA methyltransferase 3 alpha antibody (Cat#3760). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DNA methyltransferase 3 alpha antibody (Cat#3760, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of DNA methyltransferase 3 alpha expression in H9c2 cells using DNA methyltransferase 3 alpha antibody (Cat#3760, 1:2,000). Green, isotype control; red, DNA methyltransferase 3 alpha.
Western blotting analysis using DNA methyltransferase 3 alpha antibody (Cat#3760). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DNA methyltransferase 3 alpha antibody (Cat#3760, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of DNA methyltransferase 3 alpha expression in H9c2 cells using DNA methyltransferase 3 alpha antibody (Cat#3760, 1:2,000). Green, isotype control; red, DNA methyltransferase 3 alpha.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000844s ]
SELECT * FROM `goods` WHERE `code` = '3760' LIMIT 1 [ RunTime:0.000537s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000547s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000390s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000390s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 5177 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000364s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000517s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 5177 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000314s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000407s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000259s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.059749s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (4032,5044,9106,9800,8801,7397,8055,6636,5404,4209) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000794s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000477s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000236s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000495s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000228s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000376s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000405s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000313s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000209s ]
