Western blotting analysis using CD81 antibody (Cat#3770). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CD81 antibody (Cat#3770, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of CD81 expression in HepG2 cells using CD81 antibody (Cat#3770, 1:1,000). Green, isotype control; red, CD81.
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
A synthesized peptide derived from human CD81
Isotype
Mouse IgG1
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:100-1:1,000
Note
This product is for research use only.
Data
Western blotting analysis using CD81 antibody (Cat#3770). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CD81 antibody (Cat#3770, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of CD81 expression in HepG2 cells using CD81 antibody (Cat#3770, 1:1,000). Green, isotype control; red, CD81.
Western blotting analysis using CD81 antibody (Cat#3770). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CD81 antibody (Cat#3770, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of CD81 expression in HepG2 cells using CD81 antibody (Cat#3770, 1:1,000). Green, isotype control; red, CD81.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000697s ]
SELECT * FROM `goods` WHERE `code` = '3770' LIMIT 1 [ RunTime:0.000471s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000456s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000320s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000344s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 5181 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000341s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000424s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 5181 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000273s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000353s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000246s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.058650s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (7273,3837,9778,4531,9231,4934,8827,4711,5256,180) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000986s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000510s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000256s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000453s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000276s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000378s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000469s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000286s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000213s ]
