Western blotting analysis using FOS antibody (Cat#3776). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FOS antibody (Cat#3776, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716
Immunocytochemical staining of C2C12 cells with FOS antibody (Cat#3776, 1:1,000). Nuclei were stained blue with DAPI; FOS was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen
Recombinant protein of human c-fos
Isotype
Mouse IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (ICC): 1:100-1:1,000
Note
This product is for research use only.
Data
Western blotting analysis using FOS antibody (Cat#3776). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FOS antibody (Cat#3776, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716
Immunocytochemical staining of C2C12 cells with FOS antibody (Cat#3776, 1:1,000). Nuclei were stained blue with DAPI; FOS was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using FOS antibody (Cat#3776). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FOS antibody (Cat#3776, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716
Immunocytochemical staining of C2C12 cells with FOS antibody (Cat#3776, 1:1,000). Nuclei were stained blue with DAPI; FOS was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.002229s ]
SELECT * FROM `goods` WHERE `code` = '3776' LIMIT 1 [ RunTime:0.001264s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.001040s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.004140s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.003137s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 5185 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000370s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000772s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 5185 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001911s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.002986s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.003783s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.156935s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (287,4032,49,10092,6880,4231,6474,10529,140,6802) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001912s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.001010s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000363s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000569s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000724s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001234s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000530s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000651s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.001918s ]
