Western blotting analysis using CNOT2 antibody (Cat#3821). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CNOT2 antibody (Cat#3821, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of CNOT2 expression in HAP-1 cells using CNOT2 antibody (Cat#3821, 1:1,000). Green, isotype control; red, CNOT2.
Immunocytochemical staining of HAP-1 cells with CNOT2 antibody (Cat#3821, 1:500). Nuclei were stained blue with DAPI; CNOT2 stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using CNOT2 antibody (Cat#3821). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CNOT2 antibody (Cat#3821, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of CNOT2 expression in HAP-1 cells using CNOT2 antibody (Cat#3821, 1:1,000). Green, isotype control; red, CNOT2.
Immunocytochemical staining of HAP-1 cells with CNOT2 antibody (Cat#3821, 1:500). Nuclei were stained blue with DAPI; CNOT2 stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using CNOT2 antibody (Cat#3821). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CNOT2 antibody (Cat#3821, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of CNOT2 expression in HAP-1 cells using CNOT2 antibody (Cat#3821, 1:1,000). Green, isotype control; red, CNOT2.
Immunocytochemical staining of HAP-1 cells with CNOT2 antibody (Cat#3821, 1:500). Nuclei were stained blue with DAPI; CNOT2 stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000717s ]
SELECT * FROM `goods` WHERE `code` = '3821' LIMIT 1 [ RunTime:0.000491s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000396s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000328s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000356s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 5207 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000320s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000408s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 5207 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000296s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000450s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000252s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.052918s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (5114,5436,9007,6044,4327,3938,5278,6071,10120,3768) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000783s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000432s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000273s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000428s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000219s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000318s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000487s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000251s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000197s ]
