Western blotting analysis using LAMP1 antibody (Cat#3913). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with LAMP1 antibody (Cat#3913, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:400-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using LAMP1 antibody (Cat#3913). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with LAMP1 antibody (Cat#3913, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using LAMP1 antibody (Cat#3913). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with LAMP1 antibody (Cat#3913, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000791s ]
SELECT * FROM `goods` WHERE `code` = '3913' LIMIT 1 [ RunTime:0.000537s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000460s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000359s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000389s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 5262 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000502s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000681s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 5262 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000415s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000565s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000378s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.059294s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (3943,8300,9569,10408,10330,9375,3804,10323,8651,6745) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001169s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000632s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000424s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000638s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000340s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000435s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000885s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000415s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000299s ]