Western blotting analysis using NF-kB p65 antibody (Cat#4029). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NF-kB p65 antibody (Cat#4029, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of NF-kB p65 expression in HepG2 cells using NF-kB p65 antibody (Cat#4029, 1:2,000). Green, isotype control; red, NF-kB p65.
Immunocytochemical staining of HepG2 cells with NF-kB p65 antibody (Cat#4029, 1:1,000). Nuclei were stained blue with DAPI; NF-kB p65 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Immunohistochemistry was performed on paraffin-embedded mouse spleen using NF-kB p65 antibody (Cat#4029, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Immunohistochemistry was performed on paraffin-embedded human endometrial carcinoma using NF-kB p65 antibody (Cat#4029, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
