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  • Primary Antibody
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Home>Product>Primary Antibody

Keratin 7 Recombinant Rabbit mAb

#4149

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Recombinant Antibody
Western blotting analysis using keratin 7 antibody (Cat#4149). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with keratin 7 antibody (Cat#4149, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Keratin 7 expression in HepG2 cells using Keratin 7 antibody (Cat#4149, 1:2,000). Green, isotype control; red, Keratin 7.
Immunocytochemical staining of HepG2 cells with Keratin 7 antibody (Cat#4149, 1:1,000). Nuclei were stained blue with DAPI; Keratin 7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using keratin 7 antibody (Cat#4149, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Please select the specifications of the product.
Specification：
20 μL 100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    KRT7
  • Catalog：
    4149
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H

Information

Data

Information
Product Name Keratin 7 Recombinant Rabbit mAb
Aliases KRT7; Keratin 7; CK-7; SCL; K7; Keratin, Type II Cytoskeletal 7; Sarcolectin; K2C7; CK7; Keratin, 55K Type II Cytoskeletal; Type-II Keratin Kb7; Keratin 7, Type II; Cytokeratin 7; Keratin, Simple Epithelial Type I, K7; Type II Mesothelial Keratin K7; Cytokeratin-7; Keratin-7
Background

Gene Name: KRT7

NCBI Gene Entry: 3855

UniProt Entry: P08729

Application Information

Molecular Weight: Predicted, 51 kDa, observed, 51 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB9805

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC), immunohistochemistry-paraffin (IHC-P)
Immunogen A synthesized peptide derived from human Cytokeratin 7
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for 1 year.
Recommended Dilutions Western Blotting (WB): 1:2,000-1:10,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using keratin 7 antibody (Cat#4149). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with keratin 7 antibody (Cat#4149, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of Keratin 7 expression in HepG2 cells using Keratin 7 antibody (Cat#4149, 1:2,000). Green, isotype control; red, Keratin 7.
  • Immunocytochemical staining of HepG2 cells with Keratin 7 antibody (Cat#4149, 1:1,000). Nuclei were stained blue with DAPI; Keratin 7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
  • Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using keratin 7 antibody (Cat#4149, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Please select the specifications of the product.
Specification：
20 μL,100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    KRT7
  • Catalog：
    4149
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using keratin 7 antibody (Cat#4149). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with keratin 7 antibody (Cat#4149, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Keratin 7 expression in HepG2 cells using Keratin 7 antibody (Cat#4149, 1:2,000). Green, isotype control; red, Keratin 7.
Immunocytochemical staining of HepG2 cells with Keratin 7 antibody (Cat#4149, 1:1,000). Nuclei were stained blue with DAPI; Keratin 7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using keratin 7 antibody (Cat#4149, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Shopping cart (12)
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Estimated total order price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$23400
View the shopping cart Empty the shopping cart
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  • Email Address

    support@genuinbiotech.com

    sales@genuinbiotech.com

  • Office Hours

    8:30 AM - 5:30 PM EST Mon-Fri

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  145. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/public.php ( 3.56 KB )
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  147. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/facade/Request.php ( 9.20 KB )
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  149. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/facade/Route.php ( 4.70 KB )
  150. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-multi-app/src/Url.php ( 7.52 KB )
  151. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/route/Url.php ( 14.07 KB )
  152. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/head.php ( 3.43 KB )
  153. /www/wwwroot/sbywcs.dsger.com/app/web/view/product/detail.php ( 31.31 KB )
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  1. CONNECT:[ UseTime:0.000570s ] mysql:host=127.0.0.1;port=3306;dbname=sbywcs_dsger_com;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000749s ]
  3. SELECT * FROM `goods` WHERE `code` = '4149' LIMIT 1 [ RunTime:0.000514s ]
  4. SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000435s ]
  5. SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000395s ]
  6. SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000427s ]
  7. SELECT * FROM `goods_album` WHERE `goods_id` = 5487 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000431s ]
  8. SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000565s ]
  9. SELECT * FROM `goods_quotation` WHERE `goods_id` = 5487 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000287s ]
  10. SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000386s ]
  11. SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000268s ]
  12. SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.055729s ]
  13. SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (10450,7852,9971,4174,5568,133,9939,4787,5385,7333) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000824s ]
  14. SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000527s ]
  15. SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000303s ]
  16. SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000426s ]
  17. SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000239s ]
  18. SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000332s ]
  19. SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000407s ]
  20. SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000243s ]
  21. SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000222s ]
0.095624s