Western blotting analysis using early endosome antigen 1 antibody (Cat#4153). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with early endosome antigen 1 antibody (Cat#4153, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human melanoma using early endosome antigen 1 antibody (Cat#4153, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse brain using early endosome antigen 1 antibody (Cat#4153, 1:200). Antigen retrieval was done in EDTA buffer (pH 9.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HT-1080 cells with early endosome antigen 1 antibody (Cat#4153, 1:1,000). Nuclei were stained blue with DAPI; Early endosome antigen 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of early endosome antigen 1 expression in HT-1080 cells using early endosome antigen 1 antibody (Cat#4153, 1:2,000). Green, isotype control; red, early endosome antigen 1.
